Answer choices are either "high, low" or "yes, no"; For the question: "what is the level of transcription?" the answer choices are "high, low, very low"
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Answer choices are either "high, low" or "yes, no"; For the question: "what is the level of transcription?" the answer choices are "high, low, very low"
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- Which of the following describes the lac operon in E. coli when lactose and glucose is present in the culture medium? Select one: a. CAP inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. b. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. c. Catabolite activator protein (CAP) inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases. d. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases.In the lac operon system, lactose is considered the ___________, and when the gene lacI is mutated such that the protein it produces cannot bind to lactose (IS mutation), the lac operon is ________. Group of answer choices repressor; never expressed activator; constitutively expressed inducer; never expressed inducer; constitutively expressed structural gene; never expressedWhich of the following is true about the Lac operon? Group of answer choices An operon with a constitutive operator (O^C mutation) will transcribe in the presence of a super repressor It is a positive repressible operon that is influenced mainly by fluctuations in glucose levels Produces a large quantity of tryptophan in order to synthesize lactase It is found in eukaryotes and is responsible for creating an enzyme that breaks down lactose
- An E. coli cell acquires a mutation in the gene that encodes CAP. The mutation affects the DNA-binding domain of CAP and ablates the ability of CAP to bind to the CAP site. Under which condition will the genes of the lac operon be transcribed at high levels? Glucose absent, lactose absent Glucose absent, lactose present Glucose present, lactose absent Glucose present, lactose present Under any of these conditions, the lac operon will not be transcribed at high levelsWhat are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention about the role of repressor, activator, RNA polymerase in each case! A) Glucose is absent and lactose is present B) Glucose is present and lactose is present C) Glucose is present and lactose is absentThe lac operon produces enzymes that allow the bacteria E. coli to metabolize lactose, it is in an inducable operon. When lactose is present, will the repressor protein attach or leave the operon? How would this effect the production of lactose producing enzymes?
- Lac operon regulation under allolactose functions under____________ control; trp operon regulation under tryptophan functions under____________ control. Group of answer choices negative inducible; negative repressible positive inducible; negative repressible positive inducible; positive repressible negative repressible; negative inducible negative inducible; positive repressibleOn its chromosome, an E. coli cell has a genotype of lacI− lacZ+ lacY+ lacA+. It has an F′ factor with a genotype of lacI+ lacZ+ lacY+ lacA+. What is the expected level of expression of the lac operon genes (lacZ+ lacY+ lacA+) in the absence of lactose?a. Both lac operons will be expressed.b. Neither lac operon will be expressed.c. Only the chromosomal lac operon will be expressed.d. Only the lac operon on the F′ factor will be expressed.Which of the following condition(s) is optimal for expression of the lac operon? I. low glucose and formation of CAP-cAMP complex II. high glucose resulting in no cAMP III. no lactose present and a functional repressor IV. presence of lactose binding to a functional repressor
- For each of the following conditions in the Lac Operon, state whether transcription will occur or not and state why. Include all the major actors including glucose, lactose, cAMP and CAP(CPR). Key + (present) - (absent) 1) Glucose-; Lactose- 2) Glucose+; Lactose- 3) Glucose+; Lactose+ 4) Glucose-; Lactose+consider the fec operon of Staphylococcus madeupiddis, which encodes an iron citrate uptake system. The fec operon promoter, called “Pfec”, is normally off when no regulators are bound to it (like the lac operon promoter). The activator FecA senses iron citrate and brings the appropriate enzyme to Pfec to start fec transcription when iron citrate levels are high. The repressor FecB senses free iron atoms and prevents fec transcription when free iron levels are high by binding to an operator between the promoter and structural genes. Considering the fec operon described above, which mutation would result in the operon being off under all conditions? Deletion of the operator where the repressor FecB binds to the DNA Deletion of the gene encoding the repressor FecB None of these mutations would result in the operon being off under all conditions Deletion of the gene encoding the activator FecAWhat are the effects of the following conditions on Lac operon of bacteria? Please do not forget to mention about the role of repressor, activator, RNA polymerase in each case. Glucose is absent and lactose is present Glucose is present and lactose is present Glucose is present and lactose is absent