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Antibiotics should be reduced or eliminated from culture media during cell subculturing. Make it more detailed.
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.
- A culture medium that does not grow gram-positive organisms would be a differential plate enrichment plate selective plate streak plate pour plateTechnique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xo
- Why should culture media after inoculation be incubated at an optimal temperature immediately?A broth culture is initially inoculated with a single bacterial cell. For the first hour the bacteria has a generation time of 30 minutes, for the second hour the generation time has been reduced to 15 minutes, and for the third hour the generation time has been reduced to 10 minutes. At the end of the third hour how many bacteria are present in the broth culture?Topic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:
- microbiology: Aseptic Culture Techniques Lab Explain why a loop or needle is flamed before it is dipped into a tube containing a pure culture. Also, explain why it is flamed after an inoculation is completedWhich of the following is used in the sterilization of the medium?a) benmargeb) swabc) autoclaved) centrifugee) coolingDuring media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?
