Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding forfactor VIII. Not all steps will be placed.Isolate the human gene that produces factor VIII.Isolate the mRNA of the factor VIII gene.Generate cDNA of the factor VIII gene using reverse transcriptase.IncorrectInsert the factor VIII cDNA into a bacterial vector near a promoter site.Transform the vector into an E. coli bacterium.Human DNA is introduced into the bacterial cell using direct injection by asmall needle.E. coli expresses factor VIII.Answer BankThe factor VIII protein is isolated from human tissues.

Molecular cloning is used to transfer a foreign DNA into the host (bacteria) genome that is…

Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. Not all steps will be placed. Incorrect Isolate the human gene that produces factor VIII. Isolate the mRNA of the factor VIII gene. Generate cDNA of the factor VIII gene using reverse transcriptase. Insert the factor VIII cDNA into a bacterial vector near a promoter site. Transform the vector into an E. coli bacterium. Human DNA is introduced into the bacterial cell using direct injection by a small needle. E. coli expresses factor VIII. Answer Bank The factor VIII protein is isolated from human tissues.

Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. Not all steps will be placed. Incorrect Isolate the human gene that produces factor VIII. Isolate the mRNA of the factor VIII gene. Generate cDNA of the factor VIII gene using reverse transcriptase. Insert the factor VIII cDNA into a bacterial vector near a promoter site. Transform the vector into an E. coli bacterium. Human DNA is introduced into the bacterial cell using direct injection by a small needle. E. coli expresses factor VIII. Answer Bank The factor VIII protein is isolated from human tissues.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy

Section: Chapter Questions

Problem 12QP: In selecting target cells to receive a transferred gene in gene therapy, what factors do you think...

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Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. (Not all steps will be placed).
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