As a Master's student in a lab you are given a plasmid vector that has the lacZ operon system and a Ampicillin Resistant marker. You are looking at a plate with blue and white colonies. Explain how you would pick out the colonies with the insert and why you added ampicillin to your agar (Explain the lacZ operon and what resistant markers)

As a Master's student in a lab you are given a plasmid vector that has the lacZ operon system and a Ampicillin Resistant marker. You are looking at a plate with blue and white colonies. Explain how you would pick out the colonies with the insert and why you added ampicillin to your agar (Explain the lacZ operon and what resistant markers)

Name: As a Master's student in a lab you are given a plasmid vector that has
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: As a Master's student in a lab you are given a plasmid vector that has the lacZ operon system and a Ampicillin Resistant marker. You are looking at a plate with blue and white colonies. Explain how you would pick out the colonies with the insert and

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 11TYU

See similar textbooks

Similar questions

In an investigan of Lac operon experiment, the bacteria were placed in dropper bottles containing glycerol as a carbon source. Why did the culture have to be incubated for an hour in the new media? Explain in a detailed way.
Why trp operon has a higher level of expression than live operon in bacteria grown on nutrient-poor agar plates? Explain with clear reasoning
In E. coli, the trp operon is on by default, while the lac operon is off. Why do you think that this is the case?
A researcher engineers a lac operon on a plasmid but inactivates all parts of the lac operator (lacO) and the lac promoter, replacing them with the binding site for the LexA repressor (which acts in SOS response) and a promoter regulated by LexA. The plasmid is introduced into E. coli cells that have a lac operon with an inactive lacZ gene. Under what conditions will these cells produce beta-galactosidase?
A researcher engineers a lac (lactose) operon on a plasmid but inactivates all parts of the lac operator (lacO) and the lac promoter, replacing them with the binding site for the LexA repressor (which acts in SOS response) and a promoter regulated by LexA. The plasmid is introduced into E. coli cells that have a lac operon with an inactive lacZ gene. Under what conditions will these transformed cells produce beta-galactosidase?
Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA. Otherwise all other parts of the operon are functional. Circle the least number of components for an F' plasmid that will restore normal regulation and function of the lacoperon in the resulting partial diploid. (may need more than one) (a) lacI+ (b) lacO+ (c) lacP+ (d) lacZ+ (e) None, cannot be restored
What is the relation between Blue/ White Screening and lac operon?
Which of the following describes the lac operon in E. coli when lactose and glucose is present in the culture medium? Select one: a. CAP inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. b. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. c. Catabolite activator protein (CAP) inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases. d. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases.
Draw out an example of a plasmid operon set up using amp resistance (outside the arabinose operon), arabinose operon with pglo genes embedded in the arabinose digesting enzyme genes. Remember to include the operator and promotor region.
Transformation is the process where bacteria take up a plasmid from the surrounding and then express the genes into proteins. Scientists only want the genes expressed at certain times, so they control the expression using the operon system. The arabinose operon is the classic system they use to control when the genes are to be expressed by the bacteria. Arabinose is a sugar that can be added to the agar plate that will turn on the operon. If arabinose is NOT present, no genes are expressed. A. What type of operon system is this? ____(negative controlled or positive) Explain _____ B. What type of gene regulation is this____ (inducible or irrepressible) Explain: ____
A lac operon containing one mutation was cloned into a plasmid, which was introduced by transformation into a bacterium containing a wild-type lac operon. The three genes of the chromosomal operon were rendered noninducible in the presence of the plasmid. (a) What kind of mutation in the plasmid operon could have this effect? (b) Suppose the result of transformation was to cause the three plasmid lac genes to be expressed constitutively, at a high level. What type of plasmid gene mutation could have this result?
If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or glucose, how many proteins (and which ones) are bound to the lac operon? b. Without lactose, but with glucose, how many proteins (and which ones) are bound to the lac operon??