At higher magnifications, the millimeter markings may be too far apart to appear together in the field of view. If this happens, use the measurement from a lower magnification to make an estimate. For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope used in the previous question? Total Magnification x Diametermeasured Diameter = Total Magnification measured

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter15: Instrumentation In Medical Imaging
Section: Chapter Questions
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The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question.

- Answer for this question was 3mm.

 

At higher magnifications, the millimeter markings may be too far apart to appear together in the field of view. If this happens, use the measurement from a lower magnification to
make an estimate.
For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times
greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope used in the previous question?
Total Magnification x Diametermeasured
Total Magnification measured
O O O O
Diameter =
4/3mm
2/4mm
1/4
3/4mm
40 X
100 X
400 X
Transcribed Image Text:At higher magnifications, the millimeter markings may be too far apart to appear together in the field of view. If this happens, use the measurement from a lower magnification to make an estimate. For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope used in the previous question? Total Magnification x Diametermeasured Total Magnification measured O O O O Diameter = 4/3mm 2/4mm 1/4 3/4mm 40 X 100 X 400 X
1 mm
Transcribed Image Text:1 mm
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