Answered: At pH = 7, the small peptides DE, LR…

At pH = 7, the small peptides DE, LR and VF are separated using anion exchange chromotography, which of the following amino acids elutes last ? DE LR and VF elute together VE LR

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter3: The Chemistry Of Life: Organic Compounds

Section: Chapter Questions

Problem 9TYU: Which of the following levels of protein structure may be affected by hydrogen bonding? (a) primary...

See similar textbooks

Related questions

Q: In the figure below showing changing surface charges on a protein molecule, explain why the surface…

A: The isoelectric point (pI) is the pH at which the protein has no net charge. At this stage, the…

Q: Relatively hydrophobic proteins will require higher amounts of (NH4)2SO4 to precipitate.

A: Amminium sulfate precipitation is a method of protein purification by altering solubility of…

Q: Which of the following about protein denaturation is not trueA. It is always irreversible B. It is a…

A: Proteins are complex organic molecules composed of amino acid residues that are joined by peptide…

Q: There are four proteins present in a solution: Protein A (pl = 5), Protein B (pl = 3), Protein C (pl…

A: Ion exchange chromatography occurs because of the electrostatic attraction existing between charged…

Q: Give the amino acid sequence of each peptide using the fragmentsobtained by partial hydrolysis of…

A: The peptide linkage is formed between the amino group of one amino acid and the carboxyl group of…

Q: In a solution with pH ~7 most of the amino acids are form zwitterions. Name the amino acids which…

A: Amino acids: a. Amino acids are the building block of proteins that contain a carboxylic acid group…

Q: All the necessary information to form the three-dimensional structure of the protein is provided by:…

A: Proteins are formed by amino acid residues. These amino acid residues are held together by peptide…

Q: Please draw the given peptide and calculate the net charge at pH 1, 4, 8 and 12. GRNVGHEWA

A: Peptides are short chains of a range of two to fifty amino acids, connected by peptide bonds. Chains…

Q: Determine the net charge of the peptide below at pH 7 and 12. Show your solution.…

A: Given peptide sequence: Thr-Glu-Pro-Ile-Val-Ala-Pro-Met-Glu-Tyr-Gly-Lys At low pH, a peptide is…

Q: Determine the net charge (to the nearest integer) on the following peptide at pH5 AND pH 12.…

Q: V-A. Which of the following amino acids will elute first in a cation-exchange column using a buffer…

A: Ion Chromatography Separates ions and polar molecules on the basics of their affinity to the ion…

Q: A protein has an isoelectric point at 6. It is soluble at what pH?

A: Protein is a biomolecule that is also called body-building molecule. They are mainly involved in the…

Q: What determines when an amino acid ionized? i.e. Why are both Lysine (pKa 10.53) and Argenine (pKa…

A: Amino acid are the building blocks of the protein. Each amino acid has a common basic structure with…

Q: Draw the dipeptide that results when a peptide bond is formed between the two glycine molecules…

A: Introduction: Amino acid is a compound that contains an amino and a carboxyl group and a side-chain…

Q: A researcher performed an SDS-PAGE experiment (with 2- mercaptoethanol) on a mixture containing 3…

A: G

Q: Protein structures are shown below. Protein A is a natural product; whereas, protein B is as…

A: The third major protein structure is called tertiary protein structure, which is the final specific…

Q: V-A. Which of the following amino acids will elute first in a cation-exchange column using a buffer…

A: Ion exchange chromatography is a technique in which proteins are separated on the basis of charge.…

Q: 1. Please draw the given peptide and calculate the net charge at pH 1, 4, 8 and 12. GRNVGHEWA

A: A peptide can be defined as a short chain of amino acids. They are connected to each other by a…

Q: Determine the subunit composition of this protein (i.e., how many subunits are in its quaternary…

A: In electrophoresis separation of molecules is based on mass and charge. The velocity of…

Q: Which of the following is incorrect about the peptide Arg-Asp-Cys-Tyr-Gln-Arg-Glu? O a. Its…

A: The peptide given is represented in single letter codes as: RDCYQRE Its structure is:

Q: are all 20 amino acids insoluble in water? truee or false

A: There are 20 different amino acids having different characteristics and properties and based on them…

Q: Of the following amino acids, which 3 are most likely to be found on surface of a single protein.…

A: Proteins are the ultimate products of the genes. DNA is transcribed into m RNA and this is…

Q: A protein has an isoelectric point at 6. It is soluble at what pH level? A. 6 B. 10 C. 8 D. 4

A: The isoelectric point of a protein is the pH at which the net charge of the protein is zero. The…

Q: Titration Curve of Unknown Amino Acid 15 13 11

A: Amino acids contain one α-COOH and one α-NH2 groups that’s why they are called amino acids. On the…

Q: Given the amino acids below, what amino acid will elute first in a cation-exchange column. 1.…

A: Cation exchange chromatography involves the exchange of the positive cations present in the solution…

Q: The concentration of an unknown protein sample was determined as 2.3 mg/ml. Calculate the…

A: Molarity is defined as the number of moles of a solute present per liter of a solution.

Q: The use of salt bridges or hydrophobic interactions (or pockets) to stabilize interactions between…

A: Proteins: Proteins are biological polymers and mainly made up of amino acids those that are linked…

Q: The order of elution of amino acids X, Y, VW and Z in a cation exchanger eluted with a buffer of…

A: Ion exchange chromatography is used to separate molecules based on the charge. In the ion exchange…

Q: Examine the following peptide and the inserted table, and answer the 2 questions below:…

A: The net charge of peptide molecule depends upon the charge on the amino acids side chain, N-terminal…

Q: A chromatography column is used to separate four ami serine, alanine, and histidine. A cylindrical…

A: Chromatography, a group of methods for separating very small quantities of complex mixtures with…

Q: titration of which amino acid is most likely responsible for switching a protein from binding to a…

A: An ion exchange resin is a polymer that acts as a medium for ions exchange. They may be cation…

Q: The amino acid residue sequence of the hemoglobin beta subunit polypeptide would represent what type…

A: Hemoglobin, the protein present in the red blood cells is responsible for the transport of gases…

Q: Given the following protein: N-MACHKGFDSTRRKYWQNKRLCVSA|IDWQSPWKNQGILV-C The overall charge of this…

A: (1)The ionizable groups in the given peptide at pH 7 are; N-terminal : it will have +1 charge…

Q: If you incubate a protein with a single beta sheet in solution at pH = 14, the beta sheet no longer…

A: Proteins are essential for the normal functioning of the body. They are involved in providing…

Q: In a solution at physiological pH, the side chain of a polar basic amino acid bears a charge.…

A: An amino group and an acid group are present in organic molecules called amino acids. Amino…

Q: What is the percent ionization of these amino acids? 1. Aspartame at pH 5.4 2. Glutamate at pH 11.8…

A: Since we are entitled to answer up to 3 sub-parts, we’ll answer the first 3 as you have not…

Q: Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At…

A: Isoelectric point: It is also known as pI; pH at which a peptide carries no net electrical charge or…

Q: Which peptide will most strongly attach to a carboxymethyl ion exchange column at pH 7.4? O…

A: Hi! Since you have posted multiple questions and have not mentioned which to answer, we will answer…

Q: Three proteins with an overall charges of 0, -5, and -8 are passed through an anion exchange column…

A: Protein surface charge changes with pH and it is indicated by protein's isoelectric point or pI.…

Q: Protein mixture contains four proteins alpl-4).blpl-6)l.clpl-9).dlpl+11). At pH-7, using cation…

A: Cation exchanger contain (-)ve charged stationary phase and (+)ve charged mobile phase. Hence,…

Q: V-B. Which of the following peptides would be more soluble at the indicated pH? 1. (Gly)20 or…

A: There are two amino acids that are classified into 4 classes: 1. Nonpolar(aliphatic and aromatic) 2.…

Q: A protein has an asp glu/arg lys ratio of 3. Explain how would the overall charge on this molecule…

A: pKa is the negative log of acidic ion concentration and used to denote the acidity or basicity of…

Q: What does the staining of collagen on H&E slides indicate of the pH of the protein

A: Eosin is the name of several fluorescent acidic compounds which bind to and form salts with basic,…

Q: Which of the following interactions would be most impacted by placing a protein in a buffer of pH 4?…

A: The interaction between amino acids occurs for the formation of protein. When a positive charged…

Q: Denaturation is the loss of protein function from structuralchange or chemical reaction. At what…

A: Proteins have different levels of organization, which, when combined, give the functional protein.…

Q: Which of the following is incorrect concerning protein structure determination by X-ray…

A: Protein structure determination by X-Ray Crystallography: is a technique used to obtain the 3D…

Q: Draw a Cys–Gly–Asn tripeptide. Identify the peptide bond and the N- and C-termini, and determine the…

A: Draw a Cys–Gly–Asn tripeptide. Identify the peptide bond and the N- and C-termini, and determine the…

Question

Show your all calculation

At pH = 7, the small peptides DE, LR and VF are separated using anion exchange
chromotography, which of the following amino acids elutes last ?
DE
LR and VF elute together
VE
LR

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Trending now

This is a popular solution!

Step by step

Solved in 4 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

What are the charges of the following amino acids peptides at ph 14? 1. GLAVV 2. RRKKQ
Consider a short peptide with the sequence M-C-Q-L-Y-P-E-D-K. List the ionizable groups in this peptide, and the net charge of the whole peptide at pH a) 1.5, b) 7.0, and c)12.0.
You have a mixture of 4 amino acids (Q, W, E, R, T) that you want to separate using ion exchange chromatography. Which of these amino acids would bind most strongly to a DEAE anion exchange column at pH 7? Q W E R T
At pH 7.0, in what order would the following three peptides (described by their amino acid composition) be eluted from a column filled with a cationexchange polymer?Peptide A: Ala 10%, Glu 5%, Ser 5%, Leu 10%, Arg 10%, His 5%, Ile 10%, Phe 5%, Tyr 5%, Lys 10%, Gly 10%, Pro 5%, and Trp 10%.Peptide B: Ala 5%, Val 5%, Gly 10%, Asp 5%, Leu 5%, Arg 5%, Ile 5%, Phe 5%, Tyr 5%, Lys 5%, Trp 5%, Ser 5%, Thr 5%, Glu 5%, Asn 5%, Pro 10%, Met 5%, and Cys 5%.Peptide C: Ala 10%, Glu 10%, Gly 5%, Leu 5%, Asp 10%, Arg 5%, Met 5%, Cys 5%, Tyr 5%, Phe 5%, His 5%, Val 5%, Pro 5%, Thr 5%, Ser 5%, Asn 5%, and Gln 5%
what is the net charge on the peptide at PH=6? NH2-Glu-Lys-Leu-Ser-Cys-Arg-COOH
Examine the following peptide and the inserted table, and answer the 2 questions below: Thr-Glu-Pro-Ile-Val-Ala-Pro-Met-Glu-Tyr-Gly-Lys 1. Estimate the net charge of the peptide at pH 2.0. Explain. 2. Estimate the net charge of the peptide at pH 7.0. Explain.
are all 20 amino acids insoluble in water? truee or false 7
Under what pH conditions can a protein not bind to the beads in a column? pH = 7 pH = pI pH = pKa pH = -pKa
A peptide has the following sequence: Glu – His – Trp – Ser – Gly – Leu – Arg – Pro – Gly Using a table of pKa values, determine the net charge of the molecule at pH 3 pH8 pH 11
What is the overall charge on peptide AVGDWESKELLE at pH 7.0? a. -3 b. -2 c. 0 d. +1 Which of these decapeptide is the most hydrophobic? 1. ADEEEHNRQL 2. AVLKTIFYRS 3. AVLTIIFYRG 4. AVLTDNQLPK
Three proteins with an overall charges of 0, -5, and -8 are passed through an anion exchange column equilibrated in a buffer with low ionic strength and eluted with an increasing gradient of salt in a buffer. Which protein will elute last from the column? 1. Protein with no charge. 2. Protein with eight negative charges. 3. Protein with five negative charges. 4. Both charged proteins will elute at the same time.
Enter an estimate for the charge on the peptide KPIVRFDEESC at pH 8.0.