At what amount of yeast does the population in Figure 14.3 level off? YEAST POPULATION GROWTH IN A SUGAR SOLUTION OVER A 20-HOUR PERIOD 45 10 12 14 16 18 20 Hours Amount of yeast (mgioc
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- a. For an exponentially growing population, calculate μmax (units?) based on the data belowb. Calculate the specific growth rate (μ) of the population above growing at a substrate concentration of 25 mg/L according to Monod kinetics with a ks of 50 mg/L.c. Consider two genetically engineered organisms intended for use in the cleanup of hydrocarbon spills into the ocean. Bacterium alpha has a μmax of 1 d-1 and a ks of 0.1 mg/L. Bacterium beta has a μmax of 5 d-1 and a ks of 5 mg/L. If the hydrocarbon concentration is initially 1000 mg/L and the desired objective is to reduce concentrations below 0.1 mg/L what order of addition of the bacterial species is desired?For scenario 2, below write the following 1.the process that the sets of measurements represent. 2.suitable equations of the process. 3.the calculated rates and 4.the resulting units for the process as expressed by your calculation. Scenario 2 Time,0 minutes:7.13mg/L O2 Time,20 minutes:13.39mg/L O2 Algal biomass:2.3g fresh massYou have received the following measurement data from microbial culture: Time (h) Biomass (g/L) 0 1.8 1.35 1.845 2.7 1.89 4.5 1.98 6.75 2.88 9 4.23 13.5 9 18 18.9 22.5 39.78 25.2 62.1 27 84.6 28.8 93.6 31.5 101.7 33.3 102.6 Calculate the specific growth rate using the start and end points of exponential growth. Give the result (1/h) to three decimal places.
- Consider the experimental data obtained from a discontinuous alcoholic fermentation reactor shown in the table on the side, where S is the glucose concentration in the culture broth and µx is the specific growth rate of the biomass.(a)Does this bioprocess follow Monod's model?(b) What is the maximum specific growth rate?(c) What value of glucose concentration gives a specific growth rate equal to 50% of the maximum?what is k and r for each condition? K (carrying capacity): can be estimated by finding the point where the growth curve levels off. Take the average of this data point and all data collected beyond this point to estimate k (note: if the bacteria population is still growing, you can just take the last value of data for k. r: choose any two points at the log phase of growth. Then use the r formula. Just choose two waste conditions for r and k calculation so I can get the idea of how to do this, thanks.Using this data calculate growth rate r assuming the growth is exponential and use it to calculate the doubling time.
- The following carbon sources can be used for nutrition, by at least some heterotrophic organisms, except: CH3COOH CH3OH CO2 CH4 HCOOHWhich of the following agents (yeast, bacteria, and molds) do you think should be utilized to achieve a greater mass production while at the same time maintaining a cost-effective and economical production?Calculate the generation time in a growth experiment in which a medium was inoculated with 5 X 106 cells/mL of E. coli cells and following a 1 hour lag, grew exponentially for 5 hours, after which the population was 5.4 X 109cells/mL
- Pichia pastoris was inoculated at a concentration of 1.0 kg/m3 and cultivated in a medium containing glycerol as a carbon source. The following table shows the evolution of cell concentration over the time of cultivation. time (h) 0 1 2 3 5 10 15 20 25 30 40 50 Cx (kg/m3) 1,0 1,0 1,0 1,1 1,7 4,1 8,3 18,2 36,2 64,3 86,1 98,4 What is the maximum specific growth rate of these cells (h-1)?You can determine the Z-value for both organisms by calculating the temperature change required to reduce the D value by 1 log cycle. (Observations: Notice the difference in the two organisms. One has a steeper downward slope, but is offset higher. It is slightly more heat-tolerant, but slightly more susceptible to increases in temperature.) Question: Raw milk can be contaminated by several common pathogenic bacteria (i.e. Staphylococcus, M. tuberculosis) and therefore must be pasteurized. Your goal is to find the minimum temperature and time required to destroy these organisms. If you know the Z-values for staphylococcus and M. tuberculosis, do you have enough information to set the appropriate heating parameters?Yeast and lactic acid bacteria are undoubtedly the most used microorganisms in the food industry 1. State an instance where the two have collaborated in a product development 2. State an instance where yeast is desired whereas lactic acid bacteria is a nuisance 3. State an instance where lactic acid bacteria is desired whereas yeast is a nuisance