Attached is an image of EcoRI-digested plasmid samples (pBSK and pEMBL1.9); explain why the bands are found at the top, the purity of the results, and how we couple improve our results.

Q: During plasmid extraction, a student used water during the wash steps instead of ethanol. How it…

A: A plasmid extraction is a process in which  DNA is  extracted and purified. A plasmid DNA is short…

Q: If you want to run 5 μl of a PCR sample on an agarose gel, how much 6X loading dye would you prep…

A: In this question, we are given volume of PCR reaction that is 5 ul. The concentration of loading dye…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: The lysis buffer contains NaoH and SDS(sodium Dodecyl sulphate) and its used in alkaline lysis of…

Q: Comprehensively discuss the principle and applications of PCR.

A: Introduction:-  PCR, or Polymerase Chain Reaction, is a molecular biology technique for making many…

Q: A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis.…

A: From the agarose gel image, we get information about the plasmid size and the restriction sites…

Q: 3. A plasmid was cleaved with several restriction enzymes, individually and in combinations. The…

A: Restriction mapping is a technique for mapping an unknown section of DNA by breaking it down into…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: INTRODUCTION E. coli E. coli  are small gram negative motile bacilli which mainly cause urinary…

Q: Discuss why bacteria are useful in biotechnology and genetic engineering,

A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…

Q: Sequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab)…

A: In the year 1973, Walter Gilbert and Allan Maxam developed DNA sequencing method by chemical…

Q: A. Please briefly explain how Polymerase Chain Reaction works to amplify DNA.

A: As per our policy, We are answering only question 1. For the rest of the questions please repost.…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: This enzyme will be added when the cells are lysed, detroying all RNA in the sample. Why will this…

A: INTRODUCTION Plasmid Plasmid are circular DNA and into which we can insert the DNA of our interest…

Q: Please answer the following using the experiment data below. Provide the formulas or methods used…

A: Transformation efficiency is defined as the number of colonies obtained per microgram of DNA plated.

Q: This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the…

A: Ans: Recombinant DNA technology: The technique of cutting and pasting of DNA molecule with another…

Q: Fluorescence confocal microscopy (FCM) - STK38 monoclonal antibody (M01), clone 2G8- 1F3. 200 μm a.…

A: The STK38 Monoclonal Antibody from LifeSpan BioSciences is a Mouse Monoclonal antibody. This…

Q: state two ways one can extract an insert from a plasmid

A: a plasmid is typically a tiny extrachromosomal DNA molecule that is found within the cell and is…

Q: Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing

A: In this question, we have to give out line the step of extraction of DNA molecules.

Q: 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would…

A: As per our guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: The restriction digestion of plasmid DNA by using restriction nucleases is a useful tool in mapping…

Q: You will be setting up your diagnostic digest on three plasmids, the ones you began with, PGFPUV and…

A: In this question, we have to complete the table for the reaction mixture of a PCR experiment.

Q: Consider the following plasmid (size 8000 bp), with restriction sites at the positions indicated:…

A: A plasmid is determined as a very small and circular molecule of DNA, which is also double-stranded…

Q: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

A: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?

A: Following is the procedure for the separation of plasmid by the alkaline lysis method.

Q: The solid matrix that is usually used to attach the target DNA in southern blotting technique is…

A: Blotting is utilized in atomic science for the distinguishing proof of proteins and nucleic acids…

Q: During nucleic acid hybridization, the probe is labelled Question 1 options: for DNA stability to…

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: A plasmid without a selectable marker was chosen as a vector for cloning a gene. How does this…

A: Recombinant DNA technology is a technique that involves the integration of foreign DNA to a vector…

Q: You have another circular plasmid. Complete and effective digestion of this plasmid with a…

A: The restriction enzymes are endonuclease that means they can cut the DNA from the middle. They are…

Q: n. | Choose | Choose ) ent in situ hybridization nucleic acid probe cing reverse…

A: Gene expression is the process or method by which information of the genes is a passed into nucleic…

Q: During DNA profiling, DNA nucleotides hybridized with probe can be detected througha)…

A: DNA profiling is performed at many loci to be able to tell the genetic difference between different…

Q: constructing a map of the plasmid, pDiddy.   What is the smallest fragment size that the…

A: Double digestion Restriction enzymes are eye enzymes that induce cuts(cleavage) at their specific…

Q: The concentration of your RNA solution is 1500 ng/μl. How much RNA solution do you need to use when…

A: The concentration of RNA in solution can be determined by measuring absorbance at 360 nm.

Q: What are the functions of Glacial acetic acid and potassium acetate on plasmid DNA analysis

A: Plasmid isolation uses many salts and detergent for gradual separation of DNA from proteins and cell…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A: The correct answer is E) 1.6 * 1012 cells

Q: describe the two main parts of a plasmid DNA extraction and purification procedure including the…

A: Plasmid DNA extraction

Q: The transformation results below were obtained with 10 ul of intact plasmid DNA at nine…

A: Transformation Transformation is the process by which exogenous DNA is transferred into the host…

Q: We make a 1:5 dilution of our 450 ng/ul PML104 plasmid, and then use 15 of the diluted sample in a…

A: Plasmid digestion refers to the digestion of plasmid using a restriction enzyme. Restriction enzymes…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A:

Q: In assessing extracted DNA quality, what does A230 indicate? nucleic acids background reading…

A: DNA and RNA were characterized using several methods for assessing quantity, quality, and molecular…

Q: Competent E. coli cells were transformed with the PGLO plasmid. These transformed cells were then…

A: pGLO plasmid is an engineered plasmid which contains DNA sequence that enable it's replication and…

Q: . Below is a plasmid map of pBR328, describe its screening procedure. Ncol Mscl EcoRI BspEI Pvull Cm…

A: Explanation:     The plasmid map of pBR328 shows that it possesses a ColE1 ori, which is an origin…

Q: You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the…

A: A restriction endonuclease, also known as a restriction enzyme, is a bacterial enzyme that…

Q: In a molecular biology laboratory, a student obtained competent E. coli cells and used a common…

A: Kanamycin is an aminoglycoside antibiotic.

Q: 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: pBR3322, an artificially made plasmid, is shown below. EcoRI Hindi BamHI pBR322 (4.36 kb) Pull ORI…

A: Plasmid are basically extra- chromosomal structure in the bacteria other than bacteria' s own…

Q: Describe a technique you could employ to increase the concentration of the TOL plasmid.

A: Molecular biology is the study of biological macromolecules that are involved in the various…

Q: Draw a gel to represent the band shift assay result for testing the following mixtures: (1)…

A: Band shift assay is also known as electrophoretic mobility shift assay (EMSA) is the technique used…

Q: use a NanoDrop spectrophotometer to analyze plasmid DNA obtained from miniprep. What information…

A: A spectrophotometer is a device that measures the amount of light a solution has absorbed in order…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.