You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI (E), Hindll (H), and Xbal (X). You digest the plasmid DNA with each of the following combinations of enzymes and observe the results on an agarose gel. You are provided a partial plasmid map as shown below to the right.

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You are studying a new plasmid, and you digest the plasmid with three restriction
enzymes: Eco RI (E), Hindlll (H), and Xbal (X).
You digest the plasmid DNA with each of the following combinations of enzymes and
observe the results on an agarose gel. You are provided a partial plasmid map as shown
below to the right.
+18
a. What is the size of this plasmid in base pairs?
b. What is the distance in base pairs between E1 and H?
c. What is the distance in base pairs between E1 and X?
d. What is the distance in base pairs between E2 and H?
e. What is the distance in base pairs between E2 and X?
Transcribed Image Text:You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI (E), Hindlll (H), and Xbal (X). You digest the plasmid DNA with each of the following combinations of enzymes and observe the results on an agarose gel. You are provided a partial plasmid map as shown below to the right. +18 a. What is the size of this plasmid in base pairs? b. What is the distance in base pairs between E1 and H? c. What is the distance in base pairs between E1 and X? d. What is the distance in base pairs between E2 and H? e. What is the distance in base pairs between E2 and X?
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