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- In this experiment of polyphenoloxidase, why does the longer the time, the darker the color is ithe result of the unheated sample?Plating 0.1 mL of a sample diluted by a factor of 10^(-3) produced 43 colonies. What was the original concentration (OCD) in the undiluted sample?In Figure 5-3, if the concentration of bacterial cells inthe original suspension is 200/ml and 0.2 ml is platedonto each of 100 petri dishes, what is the expected average number of colonies per plate?
- plse cansomeone help determine the stain. name and duration for this bacteria."If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000Approximately how many bacterial colonies are present on plate B?
- If the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli?How might incubating plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?) If you counted 500 CFU’s in the petri dish for number 6 and 5000 CFU’s in petri dish for number 7, what would be the most justifiable explanation for this? 19) If the original sample’s concentration was 12 bacterial cells, what do you expect the count to be?
- The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?ANSWER TO QUESTION 6: 5.3x105 Using your answer from question 6, how many CFUs would be presented in 10 mL of the original culture?