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Could you perform the Ames test with a strain of bacteria that was auxotrophic for something other than histidine? How would the setup and execution of the experiment change, and why?
Note: keep it short , not too short please
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- Could you perform the Ames test with a strain of bacteria that was auxotrophic for something other than histidine? How would the setup and execution of the experiment change, and why?What is the purpose of the S-9 in the Ames test?What is the purpose of conducting a modified Furter-Meyer Test? What is the principle behind this test? How could you identify the positive result for this test?
- Why were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase's experiments?What reaction would you expect when performing a positive control in the catalase assay? What would it mean if a known catalase-positive bacteria did not produce the expected reaction?Why is Hopkin's Cole test specific for tryptophan?
- Why would it have been possible to utilize N14 in the original bacteria and only allow growing bacteria to access N15 to build new (F1) bacteria and get the same results as the original Meselson experiment?Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay experiment?The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.
- What bacteria were used for the isolation of the three enzymes (HindIII, NdeI and PvuI)? What type of ends do these restriction enzymes produce? notes are belowAn E. coli colony grew on minimal medium supplemented with arginine and leucine. However, bacteria from this colony are unable to grow and form colonies on minimal medium supplemented with arginine and methionine. What is the genotype of the bacteria in this E. coli colony?What is your understanding of the Meselson & Stahl Experiment?
