Bacterial techniques, plasmid isolation, digestion and gel electrophoresis Answer all with references 1. Explain the role of different components of LB medium. 2. Why is the media autoclaved? 3. Why is ampicillin added to the solid and liquid LB medium? 4. Why is it important to inoculate single bacterial colonies?

Bacterial techniques, plasmid isolation, digestion and gel electrophoresis Answer all with references 1. Explain the role of different components of LB medium. 2. Why is the media autoclaved? 3. Why is ampicillin added to the solid and liquid LB medium? 4. Why is it important to inoculate single bacterial colonies?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter3: Basic Hemostasis

Section3.2: Disorders Of Hemostasis

Problem 11RQ

See similar textbooks

Similar questions

transfer and isolation techniques: When getting inoculum from a slant, why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
What medium is this? What is it detecting? Why do you see the clear ring around one bacteria (how did the clearing happen)? Clearing shows the protease caseinase is present and can digest the milk protein
Rationalize the use of all the reagents used in Agarose gel electrophoresis.
Bacterial Growth lab: Each 20-minute interval, absorbance of culture was recorded. When the curve showed plateaus was the absorbance for each culture proportional to the number of bacteria present in the tubes?
Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstaining
topic: BACTERIAL ENDOTOXINS TEST Fill-in the possible effect of the procedure and give the rationale. The sample solution prepared exceeded the MVD. Effect: Rationale:
for E-coli- Describe results for Gram reaction, cell shape and arrangement (look up information) What color changes were observed for each test? Any zones of inhibition (for antibiotics only)?
microbial sensivity lab: Why does the Kirby-Bauer procedure require that the concentration of the bacteria be the same, the stage of growth constant, the growth medium the same, and the concentration or amount of drug in each disk constant?
Bacterial motility soft agar method does the test tell a microbiologist the number or distribution of the organelle?
16. Enriched media boosts the growth of a particular bacterial species. a) Trueb) False
Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings