Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done).  If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture?

Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter5: Abbreviations And Systems Of Measurement
Section: Chapter Questions
Problem 30CRQ
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Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). 
If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture?

Select one:

a.
1,300


b.
13,000


c.
None of the Above


d.
130,000


e.
1,000,000

1 ml
1 ml
A
B
99 ml
99 ml
CULTURE
0.1 ml
Transcribed Image Text:1 ml 1 ml A B 99 ml 99 ml CULTURE 0.1 ml
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