Bacterial transformation is a major concern in many medical settings. Why might health care providers be concerned? a. Pathogenic bacteria could introduce diseasecausing genes in non-pathogenic bacteria. b. Antibiotic resistance genes could be introduced to new bacteria to create “superbugs.” c. Bacteriophages could spread DNA encoding toxins to new bacteria. d. All of the above.

Bacterial transformation is a major concern in many medical settings. Why might health care providers be concerned? a. Pathogenic bacteria could introduce diseasecausing genes in non-pathogenic bacteria. b. Antibiotic resistance genes could be introduced to new bacteria to create “superbugs.” c. Bacteriophages could spread DNA encoding toxins to new bacteria. d. All of the above.

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 5RQ: What is a genetically modified organism (GMO)? a. a plant with certain genes removed b. an organism...

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Which of the following is not one of the ways that Avery’s transforming principle resembles DNA?a. It migrates like DNA in a centrifuge.b. It is not destroyed by DNA-digesting enzymes.c. It migrates like DNA in an electric field.d. It is not destroyed by protein-digesting enzymes.
A. What is the pathogen that is attacking bananas today?b. Why is this especially problematic in Africa? C. Why do we expect to lose the war with this pathogen?d. What is random mutagenesis?
DNA technology has many medical applications. Which of the following is not done routinely at present? (A) production of hormones for treating diabetes and dwarfism (B) analysis of gene expression for more informed cancer treatments (C) gene editing by the CRISPR-Cas9 system in viable human embryos to correct genetic diseases (D) prenatal identification of genetic disease alleles
Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,b
Imagine you go to the doctor because you are sick. You get antibiotics, but you hurry out of the hospital as fast as you can so you don’t pick up any antibiotic resistance genes as well. If genes were transferred directly from the environment, you could pick up a resistance gene by breathing the air. What type of gene transfer allows cells to pick up genes directly from the environment? a. Transformation b. Transduction c. Conjugation d. All of the above
a) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.
Which statement is true? a. There is no danger involved in recombinant DNA research in humans. b. Stringent safety rules make the use of recombinant DNA research impossible. c. There is no danger in releasing recombinant organisms into the environment. d. Stringent safety rules make the use of recombinant DNA research possible. e. There is no danger involved in recombinant DNA research in bacteria.
You have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligation
Which of the following is true of reverse transcriptase?a. It is required for the movement of DNA transposons.b. It catalyzes the synthesis of DNA from RNA.c. It is required for the transposition of retrotransposons.d. b and c are correct.
If you mutated once per year, there could be 40 changes, but this is unlikely. Why? (Hint: Consider what natural processes we are simulating.)
The first publications describing the successful production and intracellular replication of recombinant DNA appeared in the early 1970’s. Since then, there is a long list of breakthroughs achieved by recombinant DNA technology. But which one of the following are NOT on that list? Select more than one answer ifneeded. a) Recombinant Factor VIII protein for the treatment of hemophiliacs b) Positional cloning of over 100 human disease genes c) Bacteria producing human insulin for the treatment of diabetic patients d) Reproductive cloning of over a dozen humans
why do the bacteria grow up to the edge of the area with antibiotics, pause, then continue to grow across the area with antibiotics? A) The antibiotics caused mutations in the DNA of the bacteria, making them resistant to the antibiotics. B) The bacteria mutated as a survival response to the presence of antibiotics, enabling the bacteria to become resistant to the antibiotics. C)A few bacteria experienced random mutations in their DNA, which allowed some of them to grow even though antibiotics were present. D) Researchers added antibiotic resistant bacteria to the culture, so that the bacteria would continue to grow across the area with antibiotics.