Based on base substitution rates, which of the following is likely to be true? P(A/G) + P(A/T) OP(A/G) ≥ P(A/T) P(A/G) = P(A/T) P(A/G) ≤ P(A/T)
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- Are the following base sequences sticky or not sticky? Each piece is written 5′ to 3′.(a) TTAGC and GCTAA(b) CGTACG and CCTTCGConsider the following wild-type and mutant sequences:Wild-type ....CTTGCAAGCGAATC....Mutant ....CTTGCTAGCGAATC....The average of a number of closely related but nonidentical sequences is referred to as a(n) _____________.
- which substitution matrix would you choose for a BLASTP search? justify your choice.Is there any difference between the alignments, alignment scores, identity percentages, positives and gaps? If so, what is your explanation for the differences?Using the Dynamic Programming algorithm for pairwise local alignment we covered in class, construct the dynamic programming score table for a local alignment of the following two sequences, using the following scoring parameters: match score = +5, mismatch score = -3, gap penalty = -2.: ACGTATCGCGTATA GATGCTCTCGGAAAWhat is score of the best local alignment between these two sequences? Show the alignment of these sequences. asap
- Are the following base sequences “sticky” (complementary) or not? All sequences are written 5′ to 3′. (a) A-C-G-G-A and T-G-C-C-T (b) G-T-G-A-C and C-A-T-G-G(c) G-T-A-T-A and A-C-G-C-GGiven the following mismatch (highlighted in red), which base will be replaced after mismatch repair occurs? Mutant sequence (CH3 = methyl): CH3 I 5'-GATCTCAGGC-3' 3;-CTAGAGGCCG-5' a. G b. ADraw each of the following base pairs: A-T, G-C, and U-A
- In a genomic analysis looking for a specific disease gene,one candidate gene was found to have a single-base-pairsubstitution resulting in a nonsynonymous amino acidchange. What would you have to check before concluding that you had identified the disease-causing gene?What would be the percentage of G, C, A, and T in each column? Have to calculate the nucleotide frequency from my sequence.Shown below are several next-generation sequencing reads from a sample you have. Which of the following is the most likely candidate for the original linear piece of DNA present in the sample that created the sequence reads shown below? 5' GGGCATTA 3' 5' TACGAACA 3' 5' ATACCGGGC 3' 5' ACCGTACG 3' 5' AACATACC 3' Question 4 options: 5' ATTAACCGTACGAACATACCGGGC 3' 5' GGGCATTATACGAACAATACCGGGC 3' 5' ACCGGGCATTAACCGTACGAACAT 3' 5' AACATACCGGGCATTAACCGTACG 3' 5' GGCATTAACCGTACGAACATACCG 3' 5' ACCGTACGAACATACCGGGCATTA 3'