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Based on prior experiments, you have determined that for a culture of S. aureus an absorbance reading of 1.0 AU650 is approximately equal to 7.25 x 106 cells/mL.
How many cells should be present after 10 generations in a 1 mL culture, if the initial cell culture had an absorbance reading of 0.5? (3 Points)
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- If you added 5 mL of a 20X tocopherol to 5 mL of cell culture at a density of 2.4 x 107 cells/mL, what would the final concentration of tocepherol and cell density be?given a phage lysate with 10^11 pfu/ml and a bacterial culture with 10^8 cfu/ml, how would you obtain MOI =0.1 ?In an experiment to calculate the decimal reduction time for an Escherichia coli culture, viable cells were exposed to a constant temperature of 80°C for a set amount of time. After exposure, the remaining number of surviving cells were counted. Based on Table 1, what is the decimal reduction time?Table 1. Decimal Reduction Time for E. coli Heated to 80°C Total time of exposure (minutes): Number of Microbial Cells Present: 0 100 1 80 3 50 4 42 6.5 26 13 10 21 0
- Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?In an experiment to investigate membrane integrity, beet root sections were subjected to different chemicals. Membrane damage was estimated by the color intensity of leaked pigment. Five replicates of each treatment were prepared. Complete the following table with the absorbance values. Calculate the mean (average) and the standard Table 1: Absorbance readings of pigment leaked from damaged cells treated with two different chemicals. Replicate # Absorbance at 460 nm Chemical 2 Chemical 6 1 0.357 0.392 2 0.576 0.395 3 0.334 0.385 4 0.197 0.399 5 0.334 0.403 Average SD Calculate the Coefficient of Variance (see the appendix) for the readings of each chemical: Evaluate the precision of each and state which one is more precise:
- Strain X. spp. is a rod-shaped, anaerobic, Gram-positive bacterium with peritrichous flagella andpili that could use glucose and dye as its sole carbon source. Answer the following sections (a, band c).a) With a doubling time of 240 minutes and a starting population size of 10 bacteria cells. Howmany bacteria cells will be present after 72 hours, assuming no cell death?b) Draw the diagram and describe motility required for the bacteria to gradient concentrationof attractant (food)?"If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with a starting density of 1 x 104 cells/ml. What volume of the old culture do you add to the new 5 ml culture?
- What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?Strain X. spp. is a rod-shaped, anaerobic, Gram-positive bacterium with peritrichous flagella and pili that could use glucose and dye as its sole carbon source. Answer the following sections (a, b and c). a) With a doubling time of 240 minutes and a starting population size of 10 bacteria cells. How many bacteria cells will be present after 72 hours, assuming no cell death? b) Draw the diagram and describe motility required for the bacteria to gradient concentration of attractant (food)? c) Draw the diagram of the up flow packed-bed column biofilm reactor under continuous operation and label completely