Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will use to obtain a cell fraction that consists of mostly large cell organelles: collect pellet, collect supernatant, high-speed centrifugation for 10 min., low-speed centrifugation for 5 min, filter homogenate, grind plant tissue Step 1 grind plant tissue [Choose] collect supernatant low-speed centrifugation for 5 min high-speed centrifugation for 10-min collect pellet prind plant tissue filter homogenate Step 2 Step 3 Step 4 high-spced centrifugation fo v
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- You wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you needto centrifuge the culture at 2,000xg for 10 minutes at room temperature. The rotor of yourcentrifuge has a maximum diameter of 25.4cm and the minimum diameter of 12.4cm. At whatrpm should you spin the centrifuge for pelleting the cells?Suppose you have a suspension of HeLa cells with a cell count of 85,000 cellsper ml, and you want to prepare a suspension for your experiment that has a cellcount of 40,000 cells per ml. You need 10 mls total for your experiment. Howmany mls of cell suspension will you add to your experimental tube? How manymls of cell culture media to bring the volume up to 10 mls?Volume of cell suspension: _____________________________________Volume of cell culture media:____1-You were in the laboratory, and your instructor asked you to measure the length of 20 stained plant cells on a readymade slide; which of the following sequence of steps would be the right thing to do once you get the slide? a. Observe, measure the length of each cell under medium power using the eye piece ruler. b. Observe, draw, measure and divide the length of your drawing by the actual size of the cell. c. Observe and measure the length of each cell under high power using the micrometer slide. d. Observe, draw. and measure your drawing under medium power using a micrometer slide. e. Observe, draw, and measure your drawing under high power using your ruler. 2- you made a plant cell drawing and that drawing measured 100 mm, what is the plant cell's magnification that measured 20 um under the microscope? 3-You were interested to see a human cell under the microscope, so you went to the lab to look for a dye stain human cells you looked for a stain called…
- Please written by computer source 1. We want two T-75 flasks, each with 200,000 cells in the flask that holds 20 mL of culture medium per flask. Hemocytometer counting of cells in a 1 mm sq2 area gave an average of 12 cells from a 1:9 dilution of the cell suspension. a.) Determine the cells/mL. b.) What is the volume of your cell suspension needed to make the flasks? c.) How you would now make the flasks as a new subculture of cells for incubation?Using the attached image, explain the observed results on the NA and ECM plates. Be sure to address why each sample is either able or unable to grow on each plate and include the genotype(s) of the cells which are capable of growth.The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?
- What is happening during plasmolysis of walled plant or fungal cells? Describe the process! a. …………………………………………………………………………….. b. Plasmolysis shows that one type of molecules easily passes the plasma membrane! This molecule is the one and only ..................................................... View keyboard shortcuts EditViewInsertFormatToolsTable 12pt ParagraphWhat cell types would be able to grow on the ECM in the following situations?a) Streptomycin was not added to the ECM.b) The ECM contains thiamine.c) The ECM contains all 20 amino acids and all 5 nitrogenous nucleic acid bases. Reminder: ECM = minimal medium + glucose + has streptomycin antibioticUse the attached image to help explain the results on the NA and ECM plates. Be sure to address why each sample is either able or unable to grow on each plate and include the genotype(s) of the cells which are capable of growth.
- Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations of this separation techniqueif a student conducts an experiment cultivating a cell. at the initial time the number of cells was measured with a spectrophotometer and yielded OD = 0.01 and after 20 hours the OD increased to OD = 0,15. calculate the growth rate and doubling time.Calculate the width of all 3 cells in micrometers. Use scale. Of a 40x objective.