Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will use to obtain a cell fraction that consists mostly of liquid cytosol and cell structures of very low density: collect pellet, collect supernatant, high-speed centrifugation for 15 min., low-speed centrifugation for 5 min, filter homogenate, grind plant tissue Step 1 collect pellet Step 2 low-speed centrifugatio Step 3 collect supernatant Step 4 high-speed centrifugatic v

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Q: Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations…

A: Density gradient centrifugation is a common technique for isolating and purifying biomolecules and…

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A: The culture #1 is placed in sealed flask. So, the cells here will respire by anaerobic respiration…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell fractionation is a process that involves rupturing cells, separating and suspending cellular…

Q: DRAW IT Draw the following growth curves for E. coli, starting with 100 cells with a generation time…

A: Here, the growth curve will be drawn by plotting Time on X-axis and growth on Y-axis.

Q: Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in…

A: A Hemocytometer slide is a specially designed glass slide with Neubar's rulings in the central…

Q: describe the steps involved in primary cell dissociation protocol, assessing cell viability and sub…

A: Answer :- As we know that Cell viability examines give a readout of cell wellbeing through…

Q: How does the plant cell wall react when it is placed under hypotonic medium?

A: The plants are the multicellular eukaryotic organism of the kingdom Plantae. It is the autotrophic…

Q: Which of the following cannot be grown in artificial media and must be grown in live animals or…

A: Artificial media is a medium that is defined. This medium is chemically defined. It is a synthetic…

Q: Write instruction to make cell culture medium using DMEM consist of 10% FBS, 1% streptomycin.

A: Introduction Streptomycin is an antimycobacterial drug which is used in the treatment of…

Q: The principle of Grams staining based on the cell wall composition of bacterial cell. Justify the…

A: Gram stain divides the bacteria into two major categories Gram positive bacteria and Gram Negative…

Q: POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh)…

A: Plant osmosis means the movement of water molecules through a semi-permeable membrane from a region…

Q: Place the following in the correct order in terms of steps taken, followed by the consideration…

A: DNA extraction consists of three simple steps: lysis, precipitation, and purification.

Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…

A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…

Q: in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this…

A: Answer: Introduction: Ribosomes are big complexes of RNA and protein which do the vital task of…

Q: What are types of cell culture media?

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Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…

A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…

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A: Should plot a graph between temperature and absorbance using five parts of graph rule.

Q: Place the following in the correct order in terms of steps taken, followed by the consideration…

A: The step and consideration are arranged in a sequence.

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Q: What is the most important instrument in an animal cell culture laboratory?

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Q: Provide comparison and illustration of expected growth curves from a pure culture with the…

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Q: Provide three applications of cell suspension culture

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Q: Your first task in the lab is to learn how to culture these cells and begin expanding the culture…

A: Answer:  Yes, the cell culture media need to be changed more often than the other. The open culture…

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Q: What should be considered when preparing a monolayer cell culture?

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Q: Distance of color diameter (mm) 20 15 KMNO, 0.1M - KMNO, 1.0M - 5. 15 45 09 75 Time (minutes) 06

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Q: If a cell sample is placed in pure water, what is the concentration of the salt inside the cell?…

A: The water move inside the cell and outside the cell through the process of osmosis. If a cell is put…

Q: laboratory? explain with diagram

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Q: Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go…

A: The bacteria are classified according to their cell wall composition into Gram positive and Gram…

Q: hemocytometer

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A: In this question, we are given that 1 absorbance reading of S.aureus culture contains 5.75 x 106…

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A: The cell enumeration is important for seeding the appropriate cell density into the media flask.

Q: Importance of the variabilities in cell shape to cell use/function. with Illustrations if needed

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A: Contact inhibition is a process of arresting cell growth. When cells come in contact with each other…

Q: Why do you need to calculate for volume of media needed after computing the cells? If you are…

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Q: The following image shows part of counting chamber you used to estimate cell viability for yeast…

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Q: The microscopic preparation of the tomato pulp cells was O wet mount and temporary. O wet mount and…

A: Microscope is an optical instrument which has major application in visualizing very small to…

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A: Energy is obtained during respiration process. The chemical equation is  C6H12O6 + 6O2 → 6CO2 + 6H2O…

Q: What are the differences between “primary cell culture” and “continuous cell lines”?

A: Primary cell culture is the culture of cells directly isolated from parental tissue of interest.…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."