Below are 9 possible primer pairs. • Determine which primer pair is the best choice. • Explain why the other primers are not good choices. Calculate the Tm for each primer. Underline or highlight the region of DNA for the primer pair you chose as the best. Forward 1: Reverse 1: Forward 2: Reverse 2: Forward 3: Reverse 3: Forward 4: Reverse 4: Forward 5: Reverse 5: Forward 6: Reverse 6: Forward 7: Reverse 7: Forward 8: Reverse 8: Forward 9: Reverse 9: Answer: 5' gaaataattttgtttaactttaag 3' Tm = 5' gtttaagacaaaatagtctgg 3' Tm = 5' gtaactcagetttcaggtcg 3' Tm= 5' tctcggaatgttgcaacage 3' Tm= 5' agattagcggatcctacctg 3' 5' atgtgtaatcccagcagcag 3¹ 5' cattgattatttgcacggcg 3' 5' aaaatettctctcateegee 3' 5' tccataagattagcggatcc 3' 5' tgcaagettggetgttttgg 3' Tm= Tm= Tm= Tm= Tm = Tm= 5' gatectacctgacgcttttta 3' Tm= 5' aaataatgaattegagctcggt 3' Tm = 5'ataaaaaaategagataaccgtt 3' Tm= 5'aggtcgactctagaggate 3' Tm= 1. Best primer pair choice: 2. Why are the rest not good choices? 5'ctacctgttccatggecaac 3¹ Tm= 5' ttcgggcatggcactcttg 3' Tm= 5' tccataagattageggatce 3' Tm= 5' tctcgcatgggggaccccac 3' Tm =
Below are 9 possible primer pairs. • Determine which primer pair is the best choice. • Explain why the other primers are not good choices. Calculate the Tm for each primer. Underline or highlight the region of DNA for the primer pair you chose as the best. Forward 1: Reverse 1: Forward 2: Reverse 2: Forward 3: Reverse 3: Forward 4: Reverse 4: Forward 5: Reverse 5: Forward 6: Reverse 6: Forward 7: Reverse 7: Forward 8: Reverse 8: Forward 9: Reverse 9: Answer: 5' gaaataattttgtttaactttaag 3' Tm = 5' gtttaagacaaaatagtctgg 3' Tm = 5' gtaactcagetttcaggtcg 3' Tm= 5' tctcggaatgttgcaacage 3' Tm= 5' agattagcggatcctacctg 3' 5' atgtgtaatcccagcagcag 3¹ 5' cattgattatttgcacggcg 3' 5' aaaatettctctcateegee 3' 5' tccataagattagcggatcc 3' 5' tgcaagettggetgttttgg 3' Tm= Tm= Tm= Tm= Tm = Tm= 5' gatectacctgacgcttttta 3' Tm= 5' aaataatgaattegagctcggt 3' Tm = 5'ataaaaaaategagataaccgtt 3' Tm= 5'aggtcgactctagaggate 3' Tm= 1. Best primer pair choice: 2. Why are the rest not good choices? 5'ctacctgttccatggecaac 3¹ Tm= 5' ttcgggcatggcactcttg 3' Tm= 5' tccataagattageggatce 3' Tm= 5' tctcgcatgggggaccccac 3' Tm =
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
please answer all. I'll give thumbs up
Transcribed Image Text:Below are 9 possible primer pairs.
• Determine which primer pair is the best choice.
• Explain why the other primers are not good choices.
Calculate the Tm for each primer. Underline or highlight the region of DNA for the primer
pair you chose as the best.
Forward 1:
Reverse 1:
Forward 2:
Reverse 2:
Forward 3:
Reverse 3:
Forward 4:
Reverse 4:
Forward 5:
Reverse 5:
Forward 6:
Reverse 6:
Forward 7:
Reverse 7:
Forward 8:
Reverse 8:
Forward 9:
Reverse 9:
Answer:
5' gaaataattttgtttaactttaag 3' Tm =
5' gtttaagacaaaatagtctgg 3' Tm =
5' gtaactcagetttcaggtcg 3' Tm=
5' tctcggaatgttgcaacage 3' Tm=
5' agattagcggatcctacctg 3'
5' atgtgtaatcccagcagcag 3¹
5' cattgattatttgcacggcg 3'
5' aaaatettctctcateegee 3'
5' tccataagattagcggatcc 3'
5' tgcaagettggetgttttgg 3'
Tm=
Tm=
Tm=
Tm=
5' gatectacctgacgcttttta 3' Tm=
5' aaataatgaattegagctcggt 3' Tm =
Tm =
Tm=
5'ataaaaaaategagataaccgtt 3' Tm=
5'aggtcgactctagaggate 3' Tm=
1. Best primer pair choice:
2.
5'ctacctgttccatggecaac 3¹
Tm=
5' ttcgggcatggcactcttg 3' Tm=
Why are the rest not good choices?
5' tccataagattageggatce 3'
Tm=
5' tctcgcatgggggaccccac 3' Tm =
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 4 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education