What is the role of alcohol in extracting DNA? 1.DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. 2.DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. 3.DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. 4.DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.

What is the role of alcohol in extracting DNA? 1.DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. 2.DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. 3.DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. 4.DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.
Explain. 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.
What is the role of GelRed® in Agarose gel electrophoresis of DNA fragments? GelRed® moves down the agarose gel in response to the electric current and enables visualisation of the position of the nucleic acids within in the agarose gel. GelRed® intercalates with the Nucleic acid and, under UV light, fluoresces to enable visualisation of the position of the nucleic acids in the agarose gel. GelRed® intercalates with the Nucleic acid and enables visualisation of the position of the nucleic acids in the agarose gel. GelRed® intercalates with the amino acids in the agarose gel and enables visualisation of the position of their in the agarose gel.
Why is it important that the alcohol used in the DNA extraction is kept cold? The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA in the aqueous layer. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA in the aqueous layer.
Why is it important that the alcohol used in the DNA extraction is kept cold? 1.Helps to precipitate the DNA in the alcohol layer, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield. 2.Helps to precipitate the DNA in the aqueous layer, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield. 3.Helps to precipitate the proteins at the interface between the aqueous and alcohol layers, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield. 4.Helps to precipitate the DNA at the interface between the aqueous and alcohol layers, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield.
Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the process of extracting the DNA.
Base analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.
1. a) If one DNA segment has the following base composition, 5'-CAGTTAGTCA-3', which of the following sequences is complementary? op 1: 3'-CAGTTAGTCA-5' op 2: 3'-TGACTAACTG-5' op 3: 5'-TGACTAACTG-3' op 4: 3'-TGACTAACTG-5' b) What is the nucleotide sequence of the DNA strand that is complementary to 5'-ATCGCAACTGTCACTA-3' op 1: 5'-TAGCGTTGACAGTGATA-3' op 2: 5'-TAGTGACAGTTGCGAT-3' op 3: 5'-ATCACTGTCAACGCTA-3' op 4: 5'-UAGUGACAGUUGCGAU-3'
What is the purpose of adding table salt (NaCl) to the DNA extraction buffer? 1.Table salt dissociates into Na+ and Cl- in water The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer. 2.Table salt (NaCl) dissociates into Na+ and Cl- in alcohol. The Na+ binds to positively charged DNA and proteins, while Cl- binds to negatively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer. 3.Table salt (NaCl) dissociates into Na+ and Cl- in water. The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the alcohol layer. 4.Table salt dissociates…
During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricity
DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.
What causes the DNA to migrate from the wells toward the positive electrode? a) DNA is positively charged too, and so was attracted to the positive electrode b) DNA is negatively charged, and so was attracted to the positive electrode c) DNA is polar and forms hydrogen bonds with the positive electrodes d) DNA is electrically charged, and so cycles through an electric field The ratio of absorption at 260nm to absorption at 280nm is commonly used to assess. (Note: purity of DNA should be the right answer, but a is shown as wrong) a) The concentration of DNA and RNA. b) The purity of protein. c) Whether your DNA or RNA is contaminated Trizol. d) The concentration of protein in your sample. How many different bases are associated with RNA? a) 5 b) 4 c) 3 d) 6