Consider a separation performed on a 35.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 μm thick stationary phase. Compound A eluted at 12.61 min, compound B eluted at 13.43 min, and the unretained solvent eluted at 1.175 min. The widths at half‑height, ?1/2, of the peaks for compounds A and B are 0.142 min and 0.282 min, respectively. The number of theoretical plates for the column is calculated as ?=√NA×NB Using ?A and ?B, calculate the expected resolution for this separation. resolution:
Consider a separation performed on a 35.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 μm thick stationary phase. Compound A eluted at 12.61 min, compound B eluted at 13.43 min, and the unretained solvent eluted at 1.175 min.
The widths at half‑height, ?1/2, of the peaks for compounds A and B are 0.142 min and 0.282 min, respectively.
The number of theoretical plates for the column is calculated as
Using ?A and ?B, calculate the expected resolution for this separation.
Given that-
length of tubular column (l)= 35 mm
diameter of column (d)=0.55 mm
thickness of stationary phase =2.0 = 0.002 mm
Retention time of early eluting compound a (trA) =12.61 min
Retention time of late eluting compound a (trB) =13.43 min
Minimum possible elution time of unretained solvent (tm) =1.175 min
Peak width at half height for A, (w1/2A)=0.142 min
Peak width at half height for B, (w1/2B)=0.282 min
Now,
The number of theoretical plates are calculated as-
Here, NA and NB are theoretical plates for compounds A and B separately.
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