You transform Plasmid B into E. coli and plate onto Glucose Minimal Media + X-gal + Amp. 1. Under the conditions listed above, will rrgBA be expressed? [Select] 2. Under the conditions (media) listed above, are your recombinant cells likely to be pathogenic? [Select] 3. Is the media you are using selective? Yes 4. Is the media you are using differential? Yes 5. Is the media you are using complex? No 6. What color colonies would you expect with cells expressing this plasmid? [Select]

33) Judging by mosaic clone images above, what effect does loss of dmyc likely have on cell growth? Please briefly explain.

When plasmids are isolated from bacterial cells, they may existin a number of forms.a. List the different forms that may be found.b. Which do you think would migrate the fastest and farthest in anelectrophoresis experiment and why?

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An F' cell with a functional copy of the threonine gene on the bacterial chromosome, but with a mutated copy on the plasmid is conjugating with an F-, thr+ cell. Assuming that recombination occurs 1. F' thr+/thr- The GENOTYPE of the Donor Cell after 2. F' thr+/thr+ conjugation. 3. F- thr + The GENOTYPE of the Recipient Cell after conjugation 4. F' thr + The GENOTYPE of the 5. F' thr-/thr+ Donor Cell before conjugation. 6. F- thr-/thr+ The GENOTYPE of the 7. F' thr-/thr- Recipient Cell before conjugation. 8. F- thr-

You are investigating the activity of different cadherins in cells. You use recombinant DNA technology to express GFP-E-cadherin (green) in some L-cells (which do not normally contain cadherin) and YFP-P-cadherin (yellow) in other L-cells. Explain results you expect in three cultures containing: (1) only cells expressing GFP-E-cadherin, (2) only cells expressing YFP-P-cadherin, and (3) a mixture of GFP-E-cadherin-expressing and YFP-P-cadherin-expressing cells. Also predict how (a) knocking down the ability of the cells to synthesize fibronectin (FN) or (b) adding EGTA, which depletes free Ca2+ concentration in the culture medium, might affect the results.

7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe

Q. The diagram is given below shows conjugation between F + or Hfr donor cells with F-recipient cells. On the basis of this diagram, answer the following questions.1. How you will differentiate between an F + and an Hfr strain? 2. Among two strains, which type of strain do you expect to transfer bacterial genes to F-cells? 3. What may be the end status of both donor and recipient cells of F plasmid-mediated conjugation? 4. What may be the end result when Hfr conjugates with F- cell?

We transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp). Assume you were given competent cells of known transformation efficiency (TE).  Assume TE= 1x10[7] (note 10[7] means 10 to the power of 7). You want to have about 1000 colonies on the P-200 plate. How many nanograms of plasmid should you use in the transformation reaction? Select only one answer.   1. 0.05   2. 20.00   3. 0.50   4. 50.00   5. 200.00   6. 5.00

You have two E. coli cultures. One culture is a wild-type culture, and the other contains the lacOc mutation. But, you forgot to write labels on the tubes and have mixed them up! To help you determine which culture is which, you grow each of the cultures in growth media containing either no sugar, or just lactose. Then, you perform a beta-galactosidase assay with ONPG as your enzyme substrate. You obtain the following specific activities:   Culture 1, with no sugar: 147 units Culture 1, with lactose: 158 units Culture 2, with no sugar: 3.5 units Culture 2, with lactose: 164 units   Which culture contains which E. coli strain? Explain how you determined this.