An F' cell with a functional copy of the threonine gene on the bacterial chromosome, but with a mutated copy on the plasmid is conjugating with an F-, thr+ cell. Assuming that recombination occurs 1. F' thr+/thr- The GENOTYPE of the Donor Cell after 2. F' thr+/thr+ conjugation. 3. F- thr + The GENOTYPE of the Recipient Cell after conjugation 4. F' thr + The GENOTYPE of the 5. F' thr-/thr+ Donor Cell before conjugation. 6. F- thr-/thr+ The GENOTYPE of the 7. F' thr-/thr- Recipient Cell before conjugation. 8. F- thr-

An F' cell with a functional copy of the threonine gene on the bacterial chromosome, but with a mutated copy on the plasmid is conjugating with an F-, thr+ cell. Assuming that recombination occurs 1. F' thr+/thr- The GENOTYPE of the Donor Cell after 2. F' thr+/thr+ conjugation. 3. F- thr + The GENOTYPE of the Recipient Cell after conjugation 4. F' thr + The GENOTYPE of the 5. F' thr-/thr+ Donor Cell before conjugation. 6. F- thr-/thr+ The GENOTYPE of the 7. F' thr-/thr- Recipient Cell before conjugation. 8. F- thr-

Name: An F' cell with a functional copy of the threonine gene on the bacter
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: An F' cell with a functional copy of the threonine gene on the bacterial chromosome, butwith a mutated copy on the plasmid is conjugating with an F-, thr+ cell. Assuming thatrecombination occurs1. F' thr+/thr-The GENOTYPE of theDonor Cell after2. F'

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

See similar textbooks

Similar questions

We transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp). Assume you were given competent cells of known transformation efficiency (TE). Assume TE= 1x10[7] (note 10[7] means 10 to the power of 7). You want to have about 1000 colonies on the P-200 plate. How many nanograms of plasmid should you use in the transformation reaction? Select only one answer. 1. 0.05 2. 20.00 3. 0.50 4. 50.00 5. 200.00 6. 5.00
With regard to conjugation, a key difference between F+ and Hfrcells is that an Hfr cella. is unable to conjugate.b. transfers a plasmid to the recipient cell.c. transfers a portion of the bacterial chromosome to the recipient cell.d. becomes an F− cell after conjugation.
We transformed E coli cells with a plasmid modified to contain a 'virulence factor' which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp) Assume you were given competent cells of known transformation efficiency (TE). Assume TE= 1×10[6] (note 10[6] means 10 to the power of 6). You want to have about 1000 colonies on the P-200 plate. How many nanograms of plasmid should you use in the transtormation reaction? О 1.5.00 O 2.0.05 О 3.50.00 О 4.200.00 О 5.20.00 О 6.0.50
If we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)
Acridine orange is a chemical that inhibits the replication ofF- factor DNA but does not affect the replication of chromosomalDNA, even if the chromosomal DNA contains an Hfr. Let’s supposethat you have an E. coli strain that is unable to metabolizelactose and has an F factor that carries a streptomycin-resistantgene. You also have an F− strain of E. coli that is sensitive to streptomycinand has the genes that allow the bacterium to metabolize lactose. This second strain can grow on a lactose-containing medium.How would you generate an Hfr strain that is resistant tostreptomycin and can metabolize lactose? (Hint: F factors occasionally integrate into the chromosome to become Hfr strains, and occasionally Hfr strains excise their DNA from the chromosome to become F+ strains that carry an F′ factor.)
After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that had X-gal and ampicillin. X-Gal is often used as in indicator dye, which turns blue when metabolized by B-galactosidase protein and used to test if cloning experiments have worked. [Note look at the vector diagrams carefully] Briefly explain how you would find the bacterial cells that are transformed with the plasmid with the YFG inserted.
Draw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?
Draw a diagram/figure to explain the conjugation process (. You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?
Using the plasmid map of pBCH2.0 calculate the length of the shortest DNA fragment if this plasmid was digested with the restriction enzymes PstI and EcoRV.
You are interested in synthesizing a variant of Synechocystis sp. pcc 7803 ferredoxin having mutation (E) at its 38th residue. The gene is encoded in a pET21b vector (5.3 kbp). If you use 0.10 ng of the template plasmid (pET21b SynFd), calculate the ratio of the original to the mutated gene after 10 versus 20 cycles.
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using plasmid maps like the one below. This map shows the positions of recognition sites for a number of restriction enzymes Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzymes EcoRI and PvuII.