Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.

Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter20: Iv Medication And Titration Calculations

Section: Chapter Questions

Problem 16SST

See similar textbooks

Similar questions

During transfer of liquids using a micropipette: What would it mean if the mean is very close to the expected value but your standard deviation is high ?
For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.
A new 850 cm^2 membrane is being tested for use in a hemodialysis device. During testing, a model fluid mixture is used that contains solutes with average radii of 1 nm. In one test, the concentration of the filtrate is found to be 2.17 g/L, while the concentration on the feed-side surface is 8.52 g/L. Prior experimentation showed that the mass transfer coefficient in the device was approximately 9 × 10-4 cm/s. A) If the total filtration flow rate is measured to be 0.56 cm^3/s, what is the solute concentration in the feed solution? B) Explain how you could determine the average membrane pore radius for this membrane using this information.
In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the amount of cholesterol in the serum sample is determined as 3.11 g/L, what is the absorbance value of the sample? A. 0,280 B. 0.028 C. 0.208 D. 0.82
Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)
In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?
Propose a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.
Plot a line weaver-burk graph for Km=6.30mM and Vmax=360uM/min when an experiment has 5 tubes with concentrations of substrates of 1.0mM, 10mM, 50mM, and 100mM.
A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?
Give typed solution only Calculate the ML/HR (rate) Merrem 1 g (1g/50 mL of 0.9% NACL) IVBP Q 12 hours Infuse over 30 minutes. *Please show how you would work this using dimensional analysis, if possible. If not, it's fine if you show how you arrived at your answer another way*
When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient elution differ from Isocratic and comment on the advantages of using this type of mobile phase elution?
From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearlydefined separation between molecules? Explain your conclusions. Parameters required for calculation of coefficient (Kd) for unknown protein Volume eluted (mL) Which variable does this volume represent in the equation for Kd? Fraction with maximal DNP-Aspartate detected 36 Vt Fraction with maximal Protein detected 24 Ve Fraction with maximal Blue dextran detected 6 Vo