From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearly defined separation between molecules? Explain your conclusions. Parameters required for calculation of coefficient (Kd) for unknown protein Volume eluted (mL) Which variable does this volume represent in the equation for Kd? Fraction with maximal DNP-Aspartate detected 36 Vt Fraction with maximal Protein detected 24 Ve Fraction with maximal Blue dextran detected 6 Vo
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearly
defined separation between molecules? Explain your conclusions.
Parameters required for calculation of coefficient (Kd) for unknown protein
|
|
Volume eluted (mL) |
Which variable does this volume represent in the equation for Kd? |
|
Fraction with maximal DNP-Aspartate detected |
36 |
Vt |
|
Fraction with maximal Protein detected |
24 |
Ve |
|
Fraction with maximal Blue dextran detected |
6 |
Vo |
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