Calculate KM for the substrate SAD.

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter6: Metabolism
Section: Chapter Questions
Problem 8RQ: Which of the following is the best way to judge the relative activation energies between two given...
icon
Related questions
Question
7. An enzyme is discovered that catalyzes the chemical reaction :
SAD
+ HAPPY
A team of motivated researchers sets out to study the enzyme, which they call happyase.
They find that the kcat for happyase is 600 s -1 and carry out several additional
experiments. When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity is 9.6 µM s-1.
Calculate KM for the substrate SAD.
8. In a separate enzyme happyase experiment using [Et] = 10 nM, the reaction velocity is
measured at 3 µM s-l. What is the [S] used in this experiment?
Transcribed Image Text:7. An enzyme is discovered that catalyzes the chemical reaction : SAD + HAPPY A team of motivated researchers sets out to study the enzyme, which they call happyase. They find that the kcat for happyase is 600 s -1 and carry out several additional experiments. When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity is 9.6 µM s-1. Calculate KM for the substrate SAD. 8. In a separate enzyme happyase experiment using [Et] = 10 nM, the reaction velocity is measured at 3 µM s-l. What is the [S] used in this experiment?
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Knowledge Booster
Biochemical activities
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:
9781305389892
Author:
Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:
Cengage Learning