Can you develop a siRNA-based technology to treat COVID-19? If so, please provide the details on the stepwise design.

Q: Why are the correct primer sequences essential for successful amplification?

A: Polymerase chain reaction (PCR) is a widely used method in molecular biology that is used to make…

Q: Targeting vectors use homologoues recombination to insert transgenes at specific chosen locations in…

A: Gene targeting by homologous recombination involves exchange of genetic material between genomic…

Q: Explain the genome-wide association study (GWAS)?

A: GWAS study is important in order to determine common diseases and SNPs(single nucleotide…

Q: What is the minimum and maximum cDNA input from FFPE samples in library preparation phase to perform…

A: Next genome sequencing is quickly turning into the technique for decision for transcriptional…

Q: What is the difference between nonhomologous end-joining (NHEJ) and homology-directed repair (HDR)…

A: Genome editing can be defined as the way of of making  the specific changes to the DNA of a cell or…

Q: Like many biotechnologies, CRISPR/Cas9 was originally discovered as system that naturally occurs in…

A: Biotechnology is the branch of biology which deals with the utilisation of cells and biomolecular…

Q: What evidence demonstrates that CRISPR-Cas is an adaptive immune system defense against viruses?

A: A virus is a very small submicroscopic agent that is known for causing infections in its host…

Q: What is the difference between a Lambda  insertion vector such as Lambda gt11 and a Lambda…

A: Step 1 Cloning vectors are carriers or vehicles of desired DNA (deoxyribonucleic acid) fragments…

Q: Can you infect your dog with COVID-19

A: COVID-19 is a highly contagious infectious disease. It is caused by a coronavirus.

Q: Compare and contrast the roles of crRNA and tracrRNA in the defense process against bacteriophages…

A: Cas9 in conjunction with the engineered CRISPR sequences can be used to hunt down the codes and them…

Q: How to use gene therapy help in the cure of COVID-19?

A: Gene therapy is a treatment for the disease in which through a gene delivery vehicle the exogenous…

Q: Explain how viral vectors can be used for gene therapy, and provide two examples of commonly used…

A: The two methods of gene transfer used in gene therapy are: The viral-mediated gene transfer…

Q: What is the importance of TA within the EcoRV recognition sequence?

A: Restriction enzymes cleaves DNA sequences at sequence-specific sites thus producing DNA fragments…

Q: What primers set should we use for Sequencing on Hemaglutinin and Neuraminidase of H5N1?

A: Influenza is a viral infection that affects mainly the respiratory systems. Influenza A virus…

Q: Why are nucleoside analogs active only against replicating viruses?

A: Nucleoside analogs represent the largest class of small molecule-based antivirals, which currently…

Q: How is Covid-19 described by the scientists?

A: Covid 19 short for "coronavirus disease 2019" (the year the virus was first identified)—is a type of…

Q: What is CRISPR-Cas9? What is one advantage and one disadvantage of this technology?

A: Gene editing is a technology in which Genetic material of an organism is altered and remodelled.…

Q: Does SARS-CoV-2 conform to the central dogma of molecular biology that was coined by Francis Crick?

A: * Central dogma of  moecular biology   * It Explains the flow of genetic information from DNA to RNA…

Q: How was it experimentally proven that the spacer sequences in the CRISPR locus were the elements…

A: CRISPR-cas system provides an adaptive defence mechanism to the prokaryotes which acquires…

Q: In type II CRISPR-Cas9 systems, what intermediate interacts with the repeat sequences that are added…

A: CRISPR Cas 9 edits genes by precisely cutting DNA and then letting natural DNA repair processes to…

Q: What are the vectors used in rDNA technology? briefly explain each

A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…

Q: What is the difference between a l insertion vector such as lgt11 and a l replacement vector?

A: Vector in molecular biology is a DNA molecule which carries foreign genetic material into foreign…

Q: Describe the process of cloning a DNA fragment into the EcoR1and AluI sites of the vector pUC18. How…

A: DNA cloning is characterized as a cycle of creating different duplicates of a specific DNA. This…

Q: How do you use CRISPR to treat a patient with sickle cell anemia? Please provide a detailed…

A: In sickle cell anemia the blood cells become sickle shaped. This may result into painful blood flow,…

Q: Briefly, how can we have so many TCRs and BCRs (billions!) with only 100 genes dedicated to making…

A: The T-cell receptor (TCR) is a protein complex present on the exterior of T cells, also known as T…

Q: What does MEGA stand for and what is this assay used to determine? How to do perform the assay?

A: Many assisted reproductive techniques are widely used around the globe.

Q: With reference to the image below, discuss the process and principle involved for…

A: Molecular cloning or cloning is a technique to assemble DNA of two different organisms and directing…

Q: explain what is similar and what is different from the bacteriophages of covid-19

A: Bacteriophages or phages are the viruses that infect and kill bacteria. Bacteriophages consist of a…

Q: How can the efficiency of homologous recombination be improved when using CRISPR-Cas9, compared to…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a technique derived from…

Q: what is the importance of disclosing a COVID-19 patients identity

A: COVID -19 is a serious disease that is caused by a train coronavirus. It is also referred to as…

Q: Discuss the advantages and disadvantages of targeted vs mass control using COVID-19. ( if you can…

A: Covid-19 is a viral infection caused by a coronavirus.

Q: What is the role of the biomedical engineer in curing the COVID-19

A: Biomedical engineering applies the principles of engineering and its applications to problems…

Q: i) Suppose we want to insert the GFP sequence after the promoter of a gene X to create a fusion…

A: The CRISPR Cas9 system is a tool for cutting a DNA at a particular location. This system has 2…

Q: Describe how you would genetically design a superbugresistant to b-lactams, methicillin,…

A: Superbugs are strains of viruses, bacteria, fungi, and parasites that are resistant to most the…

Q: Which protein product of HTLV-1 functions as a transcriptional activator and is also considered to…

A: HTLV-1 is an enveloped virus that contains two strands of RNA within a protein capsid. It can infect…

Q: You receive purified mRNA encoding the peak protein of SARS Cov-2 as well as the plasmid pET32 (a…

A: DNA cloning with plasmid vector involves the addition of a foreign gene into a plasmid vector. The…

Q: Using the concept of gene therapy describe how you can design a vehicle to target SARS-CoV-2 RNA?

A: Gene therapy is a treatment for the disease, require a gene delivery system for target cells to…

Q: We have been told that some tumours in the colon are associated with microsatellite instability.…

A: MSI stands for Microsatellite instability.

Q: Does CRISPR CAS9 as a defense in bacteria only work for double stranded viral DNA? or any type of…

A: Scientists can use CRISPR to persistently edit genes in living cells and creatures and, in the…

Q: What are the potential concerns regarding the use of viral vectors in gene therapy?

A: Introduction :- Gene therapy is the process of changing the genes in your body's cells in order to…

Q: PLease explain What characteristics of plasmids makes them good cloning vectors? Discuss

A: Plasmids are small extrachromosomal DNA molecules that can reproduce independently of their…

Q: Explain why mRNA is preferred for making COVID-19 vaccines?

A: In general, a weakened or attenuated form of the pathogen is used as a vaccine to activate the…

Q: What is the functional significance of sticky ends in a cloningexperiment?What type of bonding makes…

A: Answer- Sticky ends are generated after restriction endonuclease is applied on a DNA on the…

Q: what can biomedical engineers do to help in the cure of covid?

A: Answer: COVID is the corona virus infected disease. which has been discovered in the end of 2019…

Q: What is c3435T mutataion,What is the clinical implication of c3435T mutation, what is the molecular…

A: A mutation is an alteration that  changes a nucleotide of nucleic acid. It mainly involves the…

Q: Why was the type II CRISPR-Cas9 system of S. pyogenes selected by several scientists as a model to…

A: CRISPR stands for Clustered Regularly-Interspaced Short Palindromic Repeats, is a revolutionary…

Q: What is the visibility of proteomic phenotype in COVID-19 patients?

A: The protein is defined because the set of proteins that an organism will manufacture. By…

Viral Infections

Viruses are abundantly found, minute entities that have genetic material enclosed within a protein coat. The proteinaceous material enclosing the genetic material of the virus is called a capsid. The genetic material could be either DNA (deoxyribonucleic acid) or RNA (ribonucleic acid). A virus is too minute to be looked at with naked eyes and therefore visualized with the help of a microscope. They are acellular entities that require a host for processes, such as replication, transcription, translation and multiplication. Plants, animals, and bacteria act as host wherein the virus reproduce. Viruses cannot multiply outside the host's body and thus are considered nonliving outside the host body.