Choose the basic chemical materials that are required to perform PCR. A. Two oligonucleotide primers. B. DNA fragment to be amplified. C. A heat-resistant DNA polymerase. D. All of the above
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Q: The image below represents the PCR results after electrophoresis on an agarose gel. One band is…
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Q: Which of the following is NOT needed to perform a PCR reaction? A) DNA primers B) deoxyguanosine…
A: Components required for PCR include a DNA sample which acts as a template , DNA primers, free…
Q: Link the technique to the correct enzyme.…
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Q: Explain how PCR eventually generates a discrete-sized fragment from a much longer piece of DNA.
A: Polymerase chain response (PCR) is a technique broadly used to quickly make millions to billions of…
Q: If a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10…
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Q: Contrast how gene cloning is different betweenrecombinant technology and PCR.
A: Gene cloning: It is also known as molecular cloning, it refers to the process of isolation of a DNA…
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A: Bioinformatics is the use of information technology in biotechnology for data storage, data…
Q: What components do you need to perform PCR?
A: Note: Since you have asked multiple question, we will solve first question for you. If you want any…
Q: What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a.…
A: Introduction Real-time RT–PCR is a laboratory technique, it is a nuclear-derived method for…
Q: why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only…
A: RFLP [restriction fragment length polymorphism]-- will create several fragments of DNA because DNA…
Q: Explain the role of different polymerases in PCR with their advantages and limitations. Please…
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Q: Explain how oligonucleotide primers in the Polymerase Chain Reaction work (PCR)
A: PCR or polymerase chain reaction is an in-vitro DNA amplification technique. It is used to amplify…
Q: List the ingredients and describe how a PCR reaction works
A: Polymerase chain reaction (PCR) is used for amplification of DNA. PCR is used in molecular biology…
Q: What purpose do short DNA primers serve in PCR? O They determine which sequence of DNA is copied O…
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Q: Why is the use of a heat-stable DNA polymerase important to the success of PCR?
A: The polymerase chain reaction (PCR) is a method used to amplify the deoxyribonucleic acid (DNA) of…
Q: If Susie collects her cheek cells, but accidentally dumps the cell pellet into the waste container…
A: A cell pellet is prepared from human primary cells and it is utilized in polymerase chain reaction.
Q: Explain two parameters that should be considered when designing primers for PCR.
A: PCR stands for Polymerase Chain Reaction. Using this technique, a fragment of DNA can be amplified.…
Q: When using a micro centrifuge, what should you check before starting the machine? A. That the…
A: Microcentrifuge is a type of laboratory centrifuge, which is used to separate particles in…
Q: Compare and contrast traditional PCR with quantitative PCR.
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Q: . Explain how restriction enzymes work and why they are so important to recombinant DNA technology.
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Q: What are the advantages of PCR?
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Q: In 1993, Kary Mullis won the Nobel Prize in Chemistry for his invention of the PCR process. Describe…
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Q: Describe the three steps of a PCR cycle.
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Q: use PCR to assess microbial activity, what type of PCR would you use and why?
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Q: Describe the three main steps of Polymerase Chain Reaction (PCR)
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Q: Explain the steps in recombinant DNA technology or rDNA technology
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Q: What are the steps of a PCR?
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- When using a micro centrifuge, what should you check before starting the machine? A. That the samples are balanced B. That the PCR tube caps on the samples are slightly open C. That the machines is set to 64 degrees Celsius D. That is programed for 35 cycles.List the ingredients and describe how a PCR reaction worksWould it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.
- Which of the following is involved in recombinant DNA technology? Explain. a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptaseDescribe the method of a PCR technique in which you can amplify fragments randomly? Briefly.which of the following are considered as the main tools for dna technology? a. vectors b. plasmids c. organisms d. enzymes
- What is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the aboveThe PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase (d) restriction enzymes (e) b and cChoose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copies
- Describe the three steps of a PCR cycle.A) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.Explain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.