Q: Draw the PCR machine with marking in detail
A: Polymerase chain reaction is method which is widely used to make billions of copies of the DNA…
Q: why different thermal conditions are necessary for successful PCR?
A: DNA polymerase is an enzyme that naturally polymerizes nucleotides to polynucleotide strands. The…
Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…
A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…
Q: plication of pcr technique.
A: PCR: Polymerase chain reaction.
Q: In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a)…
A: Polymerase chain reaction or PCR is a technique of formation of millions to billions of copies of a…
Q: How would I know if my PCR product is pure?
A: Polymerase Chain Reaction (PCR) is one of the most significant tools of molecular biology. It was…
Q: Which is the correct order of the PCR reaction?
A: Introduction PCR or the polymerase Chain Reaction is a molecular technique used to amplify a…
Q: What are some important safety and ethical issues raised by this use of recombinant DNA technology?
A: Recombinant DNA (deoxyribonucleic acid) technology is the process of insertion of DNA molecules…
Q: Why are entire genomes not used for DNA profiling?
A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…
Q: What is real-time PCR?
A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…
Q: What are the function of autoradiography in DNA fingerprinting?
A: Deoxyribonucleic acid (DNA) fingerprinting is laboratory technique that is used to establish a link…
Q: How do you interpret PCR?
A: Polymerase chain reaction is method of obtaining large quantities of DNA sequence by selective…
Q: What is DNA fingerprinting? Mention its applications? Help me
A: DNA is a based technology is very common nowadays. We know that the Biotechnology is the process of…
Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…
A: In the conventional amplification technique of PCR (polymerase chain reaction), the amplified…
Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?
A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…
Q: annealing temperature
A: Answer:Annealing temperature depends the Tm of the complementary regions of your primer with the…
Q: What is the purpose of the first step of PCR when it increases the temperature to 95 C.
A: The first step of PCR is to melt the DNA so that double-stranded DNA separates into single-stranded…
Q: Tell me how did you prepared the gel if the tray is 150 ml? how many milliliters of the TBE and how…
A: The answer to the first question is, 3 grams of agarose in 150 ml of TBE. *to get the second…
Q: Why different temperatures are used in a PCR reaction? Please answer at your own words.
A: The full form of PCR is polymerase chain reaction. Polymerase chain reaction is a process by which…
Q: Explain the different steps of a PCR reaction completely and in detail
A: PCR or polymerase chain reaction is technique used in laboratory to make many copies of a specific…
Q: Name four reagents used during a PCR reaction
A: Polymerase chain reaction: It is an in vitro technique that results in the exponential…
Q: PCR: The fragment size you see on the gel does not match your predicted length. What do you do
A: PCR (polymerase chain reaction) is a technique used for the selective amplification of the target…
Q: Outline the method of real-time PCR, and discuss why it is used.
A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…
Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…
A: The amplification of the DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) can be performed by…
Q: List the ingredients and describe how a PCR reaction works
A: Polymerase chain reaction (PCR) is used for amplification of DNA. PCR is used in molecular biology…
Q: Where to start with microbial complete genome analysis?
A: Genomic analysis can be described as the process of recognizing, measuring, and comparing the…
Q: Discuss the importance of components of PCR reaction in detail
A: Polymerase chain reaction (PCR) is a laboratory technique, which is used to make multiple copies of…
Q: Why is it important to wear gloves when setting up the PCR tubes?
A: PCR represents polymerase chain reaction which is an in vitro laboratory technique used for the…
Q: What are the different types of PCR and their product concepts? Site and explain the comparison…
A: Introduction :- Polymerase Chain Reaction (PCR) is a molecular biology technique for making multiple…
Q: What are the three steps of PCR?
A: PCR (polymerase chain reaction) is a technique, used for the in vitro amplifying desired DNA…
Q: Explain two parameters that should be considered when designing primers for PCR.
A: PCR stands for Polymerase Chain Reaction. Using this technique, a fragment of DNA can be amplified.…
Q: You have a 100 micromolar stock solution of dNTPs in the freezer and are setting up a PCR reaction…
A: PCR is the polymerase chain reaction. In this exact amount of reagents to be added in order to…
Q: Briefly explain Polymerase Chain Reaction (PCR) applications in clinical diagnosis.
A: Polymerase chain reaction (PCR) is a molecular biology-based laboratory method for multiplying a…
Q: What are the approximate sizes of each band and their relative brightness after clean up pcr?
A: Gel electrophoresis is a process used to separate the DNA fragments based on their size and charge.…
Q: What are the components require in a PCR reaction? And justify them? Please answer at your own…
A: PCR is the abbreviation for polymerase chain reaction which is a technique used for the…
Q: What are sonme applications of PCR?
A: According to guidelines we have to answer the first question only. so please kindly post the…
Q: Why amplify DNA using PCR before doing electrophoresis? Why not just take your DNA sample and…
A: The polymerase chain reaction (PCR) is a typical laboratory technique for making multiple copies of…
Q: What is the final volume of the individual PCR reactions we are making?
A: Polymerase chain reaction (PCR) is an in vitro method of making large number of copies of desired…
Q: What is the difference between PCR and real time PCR? and What happens during PCR? Write in detail.
A: Molecular biology:It is the study of living things at a molecular level. It strives to understand…
Q: how do you prepare 200 ml of 1.5 agarose gel in PCR?
A: Agarose is an uncharged, inert substance that does not bind to or react with molecules. Agarose is…
Q: Identify three items (aside from water) that must be used in a Polymerase Chain Reaction (PCR) mi
A: Items that must be used in a Polymerase Chain Reaction (PCR) mixture are- MgCl2. dNTPs. Taq…
Q: What determines the size (length) of the primary PCR product? What might a successful gel check of…
A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene of…
Q: what are the advantages and disadvantages of Gel electrophoresis in DNA analysis
A: Introduction :- Electrophoresis is a technique used in laboratories to separate DNA, RNA, and…
Q: at is the purpose of the PCR technique in Covid testing?
A: The question is all about the PCR technique i.e polymerase chain reaction technique. It is the…
Q: Briefly describe the functions of the components of a typical PCR reaction.
A: Polymerase chain reaction, or PCR, is a procedure to make many duplicates of a particular DNA…
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- 1: what actually happens in lab for COVID PCR. 2: How PCR for COVID testing is done from start to finish. 3: explain how PCR is done to detect COVID from patients. 4: how/what is done for COVID PCR.1. What are the reaction components, and what equipment do you need for PCR?2. What can be possible source of DNA for PCR?3. What are some uses for PCR?The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)
- What is PCR? why is it important for the manufacture of drugs? How is it used in forensic science?1. You are working as a CSI analyst. You collected DNA at a crime scene that is from the suspect but do not have enough to run a test to determine who the suspect is. Please discuss what you would do to attain more of the DNA and how to test the DNA to determine who the suspect is.2. Please discuss how the bacterium Agrobacterium tumefaciens normally functions. Please also discuss how this bacterium can be used to help plants attain genes to help fight off insects and herbicides.What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one person
- Briefly explain the functions of the four procedures you learned about in this lab: DNA extraction PCR Gel electrophoresis DNA sequencing and analysisIf Susie collects her cheek cells, but accidentally dumps the cell pellet into the waste container in step 4 of the DNA collection procedure, and doesn't realize it, what is the problem with her sample, moving forward, if any? A Susie has no cells in her sample, so no DNA can be prepared from her sample. The PCR reaction will not contain template DNA. B No problem. Susie can expect the PCR reaction to work fine.1. What is DNA cloning of animals and plants? 2. What is the importance of DNA cloning? 3. Give example or scenario that involves DNA clining (could be taken on the internet). please answer 1,2,3. thank you
- Can you help me with this question, please? What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personBrenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break? A. Obtaining and handling DNA sample without wearing googles or gloves B. Improper use of human DNA samples C. Violating Patient Confidentiality D. Disposing of bio hazardous material in a regular trash1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total volume into an agarose gel. What was the original concentration of the loading dye? a. 4x b. 5x c. 6x d. 7x e. None of the above 1. b)A technologist adds 6uL of 5x loading dye to a DNA sample before adding the total volume into the agarose gel. How much DNA sample was combined with the loading dye? 12uL 16uL 20uL 24uL 28uL