asap please genetics Choose the best options, by dragging and dropping the colour coded text boxes below, that fill in the blanks correctly inthe right order, considering the entire statement is related to only one operon:operon, and it hasA(n)formattenuatorsecond4is involved in the regulation of thedifferent combinations of hairpin structures. Thewhich result in the stalling of thetrpoperator3inducerLac repressor Trp repressorfirstTrp codonswhen the2tryptophanlacribosomethirdregions thatregion has tandemconcentration is low.RNA polymerase

Operon is transcription unit of prokaryotic genome with consist of many contiguous structural genes…

Choose the best options, by dragging and dropping the colour coded text boxes below, that fill in the blanks correctly in the right order, considering the entire statement is related to only one operon: A(n) is involved in the regulation of the operon, and it has form different combinations of hairpin structures. The attenuator second 4 which result in the stalling of the trp operator 3 inducer Lac repressor Trp repressor first Trp codons when the 2 tryptophan lac ribosome third regions that region has tandem concentration is low. RNA polymerase

Choose the best options, by dragging and dropping the colour coded text boxes below, that fill in the blanks correctly in the right order, considering the entire statement is related to only one operon: A(n) is involved in the regulation of the operon, and it has form different combinations of hairpin structures. The attenuator second 4 which result in the stalling of the trp operator 3 inducer Lac repressor Trp repressor first Trp codons when the 2 tryptophan lac ribosome third regions that region has tandem concentration is low. RNA polymerase

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter10: Control Of Gene Expression

Section: Chapter Questions

Problem 2SQ

See similar textbooks

Similar questions

. In an effort to determine the location of an operator sitefor a negatively regulated gene, you have made a seriesof deletions within the regulatory region. The extent ofeach deletion is shown by the line underneath the sequence, and the resulting expression from the operon(i = inducible; c = constitutive; − = no expression) isalso indicated.... GGAT C T T AGCCGGCTAACATGATAAATATAA......C C T AGAATCGGCCGA TTGTA C T A TTT ATAT T ...1 i2 –3 c4 –5 ca. What can you conclude from these data about thelocation of the operator site?b. Why do you think deletions 2 and 4 show no expression of the gene?
You are studying an operon containing three genesthat are cotranscribed in the order hupF, hupH, andhupG. Diagram the mRNA for this operon, showingthe location of the 5′ and 3′ ends, all open readingframes, translational start sites, stop codons, transcription termination signals, and any regions thatmight be in the mRNA but do not serve any of thesefunctions.
The symbols a, b, and c in the table below represent theE. coli lac system genes for the repressor (I ), the operator(O) region, and the β-galactosidase (Z), although notnecessarily in that order. Furthermore, the order inwhich the symbols are written in the genotypes is notnecessarily the actual sequence in the lac operon.Activity (+) or inactivity (−) of Z geneGenotype Inducer absent Inducer presenta- b+ c+ + +a+ b+ c- + +a+ b- c- - a+ b- c+/a- b+ c- + +a+ b+ c+/a- b- c- - +a+ b+ c-/a- b- c+ - +a- b+ c+/a+ b- c- + +a. Which symbol (a, b, or c) represents each of the lacgenes I, O, and Z ?b. In the table, a superscript minus sign on a gene symbolmerely indicates a mutant, but some mutant behaviorsin this system are given special mutant designations.Using the conventional gene symbols for the lac operon,designate each genotype in the table.
One of the main lessons of this chapter is that severalbacterial genes are often transcribed from a singlepromoter into a large multigene (polycistronic) transcript. The region of DNA containing the set of genesthat are cotranscribed, along with all of the regulatoryelements that control the expression of these genes, iscalled an operon.a. Which of the mechanisms in the following listcould explain differences in the levels of themRNAs for different operons?b. Which of the mechanisms in the following listcould explain differences in the levels of theprotein products of different genes in the sameoperon?i. Different promoters might have different DNAsequences.ii. Different promoters might be recognized bydifferent types of RNA polymerase.iii. The secondary structures of mRNAs might differ so as to influence the rate at which they aredegraded by ribonucleases.PROBLEMSProblems 575a. Why did the mating with a nonlysogenic recipientresult in phage growth and release, but the infectionof a…
Suppose that you have isolated a mutant strain of E. coli inwhich the lac operon is constitutively expressed. To understandthe nature of this defect, you create a merozygote in which themutant strain contains an F′ factor with a normal lac operonand a normal lacI gene. You then compare the mutant strain andthe merozygote with regard to their β-galactosidase activities inthe presence and absence of lactose. You obtain the followingresults:
The activator (V protein) that is present in the bacterial cell is normally phosphorylated, but only binds to the A site in the operon when it is not phosphorylated. What operon-independent mechanism can regulate the binding of (and also ultimately regulates the removal of) protein V to the A site? Describe this binding/removal mechanism in detail – useappropriateterminologythatwasdiscussedinlecture. Aspartofyouransweraboutthisactivatorproteinbindingto (and then detaching from) the A site of the operon, be sure to address what are the results of this type of control in terms of function of the operon.
Six strains of E. coli (mutants 1–6) that had one of thefollowing mutations (i–vi) affecting the lac operonwere isolated.i. deletion of lacYii. ocmutationiii. missense mutation in lacZiv. inversion of the lac operon (but not an inversion ofthe lacI gene)v. superrepressor mutationvi. inversion of lacZ, Y, and A but not lacI, P, oa. Which of these mutations would prevent the strainfrom utilizing lactose?b. The entire lac operon (including the lacI gene andits promoter) from each of the six E. coli strainswas cloned into a plasmid vector containing an ampicillin resistance gene. Each recombinant plasmidwas transformed into each of the six strains to create partial diploids. In analysis of these strains, mutant 1 was found to carry a deletion of lacY, so thisstrain corresponds to mutation i in the list above.Which of the other types of mutations would be expected to complement mutant 1 in these partial diploids so as to allow lactose utilization?c. In part (b), each strain was plated on…
The figure at the beginning of this chapter showsthe binding of both a Lac repressor tetramer and aCRP-cAMP complex to the regulatory region of thelac operon.a. What is the key feature of a regulatory protein suchas the Lac repressor or CRP that allows it to regulate specifically the genes or operons it is supposedto control?b. On the figure, show the positions of the followingcomponents: (i) A Lac repressor monomer; (ii) a Lacrepressor dimer; (iii) all four DNA binding domains ofthe Lac repressor tetramer; (iv) a single helix-turnhelix motif; (v) the o1 part and either the o2 or o3 partsof the lac operator (assume the operon would be transcribed from right to left on the figure); (vi) themultimerization domains of the four Lac repressormonomers; (vii) an inducer-interacting domain; (viii)the CRP-cAMP complex; and (ix) a DNA loop.c. What is the physical basis for the formation of theDNA loop shown in the figure?d. On the figure, show the position of two axes ofsymmetry in the sequence…
A mutant strain has a defective lac operator site that results in theconstitutive expression of the lac operon. Outline an experimentyou would carry out to demonstrate that the operator site mustbe physically adjacent to the genes that it influences. Based onyour knowledge of the lac operon, describe the results you wouldexpect.
Attenuation of the trp operon is regulated by the efficiency of translation of the trp leader (trpL).A scientist mutates the aminoacyl tRNA synthetase responsible for charging tRNATrp, resulting in1/100 the normal amount of charged tRNATrp. How would this mutation impact attenuation ofthe trp operon? Explain why.
You have isolated two different mutants (reg1 andreg2) causing constitutive expression of the emu operon (emu1 emu2). One mutant contains a defect in aDNA-binding site, and the other has a loss-of-functiondefect in the gene encoding a protein that binds tothe site.a. Is the DNA-binding protein a positive or negativeregulator of gene expression?b. To determine which mutant has a defect in the siteand which one has a mutation in the binding protein, you decide to do an analysis using F′ plasmids. Assuming you can assay levels of the Emu1and Emu2 proteins, what results do you predict forthe two strains (i and ii; see descriptions below) ifreg2 encodes the regulatory protein and reg1 is theregulatory site?i. F′ (reg1−reg2+emu1−emu2+)/reg1+reg2+emu1+emu2−ii. F′ (reg1+reg2−emu1−emu2+)/reg1+reg2+emu1+emu2−c. What results do you predict for the two strains(i and ii) if reg1 encodes the regulatory proteinand reg2 is the regulatory site?
Resection of a bacterial chromosome in which genes for the enzymes of of a particular metabolic pathway are clusteredtogether in a functional complex under coordinate control is called operon activator promotor repressor operator