Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it is packed into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull. The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and a silica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume the same type of binding forces between the components and the stationary phase are present in both paper chromatography and column chromatography.)

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter29: Supercritical Fluid Chromatography And Extraction
Section: Chapter Questions
Problem 29.5QAP
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A two-component mixture is analyzed by paper TLC. Component A is
more soluble in the mobile phase than component B. The following
result is obtained. Calculate the R for each component and label the
identity of each spot. 2
Prelaboratory Assignment for Paper Chromatography
0
-Sporting an
on 1 by g
Transcribed Image Text:A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase than component B. The following result is obtained. Calculate the R for each component and label the identity of each spot. 2 Prelaboratory Assignment for Paper Chromatography 0 -Sporting an on 1 by g
. Column Chromatography works by the same principle as TLC however
the silica gel is not plated on a plate but it is packed into a glass (or
plastic column) and the eluting solvents runs down a column by
gravitational pull.
The mixture from question 1 (above) is analyzed by column
chromatography using the same mobile phase and a silica gel stationary
phase. Which component has the shorter retention time? Explain your
answer. (Assume the same type of binding forces between the
components and the stationary phase are present in both paper
chromatography and column chromatography.)
Transcribed Image Text:. Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it is packed into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull. The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and a silica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume the same type of binding forces between the components and the stationary phase are present in both paper chromatography and column chromatography.)
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