Compare and contrast the assembly of genomes using Sanger and next-generation sequencing (NGS) approaches

Q: explain the concept behind capillary electrophoresis in DNA quantification.

A: Introduction: Capillary electrophoresis is a technique that is utilized to separate complex mixtures…

Q: What is a database? What types of information are stored within adatabase? Where does the…

A: The complete set of chromosomes present in the organism is known as the genome. Bioinformatics…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: Describe the components vital for DNA sequencing with the Sanger method

A: DNA sequencing: The process of identifying the correct order of the four nitrogen bases (adenine,…

Q: Describe how you would clone a gene by positional cloning.Explain how a (previously made) contig…

A: The molecular cloning is also known as gene cloning. It is the process that the involves isolation…

Q: Write down all possible outcomes for template ATGCCTAAGTTTCCCTAT sequencing

A: Deoxyribonucleic acid (DNA) is the macromolecule that stores genetic information. It is present in…

Q: Genome is a word used to refer to

A: Genes are the unit of hereditary or passing information from one generation to another. The term is…

Q: compare  and contrast the bacterial and eukaryote genome.

A: All living organisms, prokaryotes as well as Eukaryotes have their genetic material in the form of…

Q: Compare and contrast a genomic library with a cDNA library.

A: The word library in biology refers to the DNA collection obtained via molecular cloning. Three types…

Q: Using the new sequencing platforms as examples discuss how DNA sequencing technologies have evolved…

A: Dna sequencing g is highly essential for the researchers and the scientists to understand different…

Q: Sanger sequencing Write a precise and accurate differential report on the above sequencing…

A: DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a…

Q: Contrast how gene cloning is different betweenrecombinant technology and PCR.

A: Gene cloning: It is also known as molecular cloning, it refers to the process of isolation of a DNA…

Q: list and explain two strategic between the shotgun sequences/ assemble strategy for whole genome…

A: Genomic Sequencing is a process where whole genome of an organism is sequenced. With the help of…

Q: What are three benefits Ion torrent sequencing has over sanger sequencing

A: DNA is an important biomolecule in the cell as it is the genetic material for most of the organisms…

Q: Methods of IS DNA Sequencing

A: DNA sequencing is the process of getting the sequence of nucleotides in a DNA. Every DNA consists of…

Q: Compare the information that can be obtained fromgenomic and cDNA libraries

A: Deoxyribonucleic acid (DNA) libraries are constructed by scientists using genetic engineering…

Q: Compare and contrast bacteria and eukaryotic genomes

A: Bacteria are prokaryotic organisms that shows differences with the eukaryotic organisms in several…

Q: Briefl y summarize the steps involved in DNA sequencing and describe its applications.

A: DNA Sequencing is the process or method which determines the order of the four nucleotides bases…

Q: Compare and contrast the use of PCR and gene cloning for amplifying DNA fragments. What are the…

A: Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy of…

Q: Describe two ways in which genome database information is used to study human disease genes.

A: The genome encodes all the information of the deoxyribonucleic acid, the macromolecule containing…

Q: To discuss: The value of genome projects.

A: Recombinant DNA technology (rDNA technology) is extremely useful for analyzing a species' entire…

Q: What information can be gathered by using DNA gel electrophoresis? Describe the steps in the process…

A: Gel electrophoresis is a molecular biology technique heavily used in laboratories. Electrophoresis…

Q: fill ups • Genome browsers show the arrangement and structure of_____ within RefSeq genomes.

A: A genome browser is a tool in bioinformatics which is used to display biological information in…

Q: Prepare an X µL PCR reaction mixture and set thermal conditions for gene amplification?

A: PCR is a strong amplification technique that can produce a large amount of a particular DNA segment…

Q: Discuss the concept of the use of DNA in genome identifi cation.

A: DNA is a substance consisting of 2 polynucleotide chains that coil around each other to form a…

Q: Describe FISH analysis and its application in findingspecific DNA sequences in a chromosome.

A: The study of the structure and function of the chromosome to understand the pattern of inheritance…

Q: Describe the outcome of a chain-terminator sequencing procedure in which (a) too little ddNTP is…

A: Sanger DNA sequencing is also known as the sequencing process of chain-termination. In addition to…

Q: Which statement BEST differentiate metagenomics from proteomics? a The material used in…

A: 'Omics' is the suffix added to frequently describe a word that refers to the field of study in…

Q: Define repetitive sequence and explain how this type ofsequence affects genome sizes

A: DNA molecules are made up of polynucleotide chains. These chains are coiled around each other and…

Q: describe in general terms how a researcher can map the genome of a newly discovered organism.

A: Genome mapping is the process of finding the locus of genes and establishing the distance between…

Q: Describe how genome-wide association studies have led to new understandings about the structure and…

A: Systems biology is a method for comprehending complex biological systems. It necessitates the…

Q: Make a table comparing the advantages and disadvantages of PCR and gene cloning for amplifying DNA…

A: PCR or polymerase chain reaction is a method by which small fragments of DNA are used as a template…

Q: Describe the process for shotgun sequencing of a genome. Practice aligning the two sets of sequenced…

A: Two questions have been asked. first question has been answered. To get the answer for your second…

Q: Review DNA sequencing and cloning tools. Match term and its description. main technologies for…

A: DNA sequencing is the process of identifying and writing the code of the whole genome in exact…

Q: Provide five advantages of Next Generation Sequencing? and explain each of these advantages.

A: Here I will discuss about the advantages of next generation sequencing.

Q: Explain how Sanger sequencing works

A: Genome sequencing: genome means total gene content present in the organism and sequence means to…

Q: The FISH technique allows researchers to localize specificDNA sequences with respect to chromosomal…

A: Fluorescence in situ hybridization (FISH) is cytogenetic technique used by researchers to detect and…

Q: Compare and contrast the chemical (maxam Gilbert) and enzymatic (sanger) methods of DNA sequencing.

A: DNA sequencing is the method of determining the order of nucleotide bases, adenine, guanine,…

Q: Explain why exome sequencing can be almost as valuable as genome sequencing. (Explain in your own…

A: Exome sequencing is one of the molecular tool to analyze the genes encoding for protein in entire…

Q: Compare and contrast PCR and the cycles used in Sanger sequencing

A: DNA sequencing is the process of determining the sequence of nucleotide bases (As, Ts, Cs, and Gs)…

Q: Describe how Ion torren method of sequencing works in detail.

A: Thermo Fisher Scientific's Ion Torrent technology brings a whole new method to Next Generation…

Q: List the steps to make a genomic library. What steps would change if you wanted to make a cDNA…

A: You have asked two questions. The first one has been answered. To get the answer for the second…

Q: Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have…

A: In conventional methods of gene expression analysis, Northern blot tests, Serial Analysis of Gene…

Q: List down and briefly describe three other sequence alignment tools other than BLAST used in…

A: BLAST can rapidly align and compare a query DNA sequence with a database of sequences, which makes…

Q: Describe two things that you can learn by searching a computerized database for sequences that have…

A: DNA is the genetic material in all living organisms. DNA is double stranded and the strands are…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.