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What is the correct chronological order of the initiation of
A. Hydrogen bonds between complementary base pairs are broken at origin of replication
B. Primase binds to the site of origin.
C. DNA polymerase binds to the template strand.
D. An RNA primer is synthesized.
E. Incoming deoxynucleoside triphosphates are added to the growing strand.
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- Which describes the role of primase during replication? a. It catalyzes the formation of phosphodiester bonds using NTPs as substrates. \ b. It coordinates synthesis of the leading strand and the lagging strand. c. It functions as a holoenzyme that polymerizes in the 3’→ 5’ direction. d. It uses an exonuclease activity to remove incorrect nucleotides.What is DNA polymerase? a.An enzyme that carries out DNA replication b.Short, single strand of DNA that base-pairs with a specific DNA sequence c.An enzyme that corrects mutations that arise during the replication of DNA d.An enzyme that seals any gaps that remain between bases of replicating strands of DNAWhat is the function of Ligase during DNA Replication a Releases tension on the DNA as it unwinds b Puts down RNA primers so replication can continue c Binds Okazaki Fragments into a continuous DNA strand d Binds to single stranded DNA to protect it from degradation
- What is the function of resolvase in recombination? a. It unwinds double-stranded DNA. b. It allows a single DNA strand to invade a DNA helix. c. It displaces one of the original DNA strands during branch migration. d. It cleaves the Holliday structure.What is DNA polymerase? A. An enzyme that corrects mutations that arise during the replication of DNA B. An enzyme that seals any gaps that remain between bases of replicating strands of DNA C. Short, single strand of DNA that base-pairs with a specific DNA sequence D. An enzyme that carries out DNA replicationHow is synthesis terminated in DNA Replication? a. DNA Polymerase runs out of template b. DNA Polymerase reaches an end site c. DNA Polymerase reads a stop codon d. DNA Polymerase adds a Poly-A Tail to the new daughter strand e. DNA Polymerase synthesizes a terminator sequence
- Without the action of DNA ligase, cells would not be ableto complete their replication. What is the function of DNAligase?(a) Unzips the DNA double helix(b) Stabilizes single-stranded DNA(c) Binds DNA sequences together to generate acontinuous strand(d) Proofreads the replication process(e) Creates an RNA copy of the DNAWhich describes the role of primase during replication? It catalyzes the formation of phosphodiester bonds using NTPs as substrates. It coordinates synthesis of the leading strand and the lagging strand. It functions as a holoenzyme that polymerizes in the 3’→ 5’ direction. It uses an exonuclease activity to remove incorrect nucleotides.A drug that inhibits the DNAa protein is added to a culture of E. coli cells. What is the first step of DNA replication that will be impaired by this drug? PICK ONE ANSWER: A. Keeping parent DNA strands separated B. Releasing tension generated by supercoiling C. Activating the origin of replication D. Making primers E. Forming phosphodiester bonds
- Why is DNA replication considered semiconservative? A. Both strands of each replicating DNA molecules are conserved. B. One molecule consists of the old strands and the other DNA molecule is entirely new. C. One strand of each replicating DNA molecule is conserved and the other strand is newly synthesized. D. Both strands of each replicating DNA molecule are new.Draw a diagram of DNA replication that has the double-stranded DNA with the replication bubble, has all 6 enzymes, two leading strands including their primers, and demonstrates how the lagging strand is replicated.The Meselson and Stahl experiment used a density label toa. determine the directionality of DNA replication.b. differentially label DNA and protein.c. distinguish between newly replicated and old strands.d. distinguish between replicated DNA and RNA primers.