cterium-mediated plant transformation is a method used to create transgenic plants cterium tumefaciens, a soil bacterium works exceptionally well as a natural geneti- r. Describe briefly the creation of a transgenic plant using A. tumefaciens with on.
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- What is the purpose of the activity “Transgenic Manipulation?”IRRI is currently in the process of developing a new variety of transgenic rice called Golden Rice. What is a transgenic organism? Describe what makes Golden Rice a transgenic organism. Why was Golden Rice developed?what are some of the benefits of producing transgenic crops for farmers and for consumers? List at least three benefits.
- Transgenic bacteria can be used to make an alanine rich (GM) plant. Explain how bacteria can be used to produce large amounts as a cheap source of protein. Your explanation will include the role of: Restriction enzymes, plasmids, recombinant DNA, and bacteria.What is the difference between a transgenic plant and a plant produced through selective breeding?Your supervisor asked you to insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.
- Outline the steps in making transgenic plants using Agrobacterium tumefaciens.Which of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin production An SNP is found associated with individuals who have diabetes. Which statement is true? A. All individuals with the SNP will have diabetes. B. The SNP codes for a faulty protein that causes the individual to have diabetes. C. To identify the SNP in more individuals, they will need to have their genome sequenced. D. The SNP may be linked to a gene that contributes to diabetes.Briefly and in simple terms, describe how CRISPR could be utilized in treating genetic conditions such as Batten disease.
- Explain why phenol:chloroform is used in the isolation of genomic DNA (What is the end result and what must you do for the desired effect)Describe the possible ethical or biological problems associatedwith eating peanuts from an engineered plant or pork from a transgenicpig.When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible to perform a "blue-white screen" to determine which bacteria have taken up a plasmid into which a DNA fragment as been inserted, as opposed to those that have taken up just reclosed plasmid vector, by growing the transformed cells on nutrient agar plates containing the artificial b-gal substrate X-gal. Will bacteria that have taken up a plasmid into which a DNA fragment has been inserted form a blue colony or a white colony when grown on this medium? Briefly explain why these bacteria would form a colony of the color you chose.