describe an appropriate and most popular method for viral detection (quick and reliable) that could be adopted at the microbiology laboratory of the North-West University for detection of COVID19 infection
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describe an appropriate and most popular method for viral detection (quick and reliable) that could be adopted at the
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- What approach can be used to enumerate the virus MNV-1 (murine norovirus) if you want to test a disinfectant's efficacy against the virus?Based on the available data, describe an appropriate and most popular method for viral detection (quick and reliable) that could be adopted at the microbiology laboratory for detection of COVID19 infection. Highlight the link between method reliability and principles involved. In addition, limitation of this approach should also be emphasized .What is the importance of aseptic techniques and sterilization in dealing with SarsCoV 2 Virus (COVID 19 agent) throat swab samples from suspected patients?
- Which of the following methods can be applied to detect the infectious virus Immuno-electron microscopy Plaque assay Western blot Hemagglutination inhibition Quantitative PCRDiscuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods 5..1 ELISA 5.2 Restriction Fragment Length Polymorphism 5.3 Polymerase chain reactionExplain the main defects of using animal models for viral growth and cultivation.
- What are the significance and correlation of antibody testing and antifen testing under quality control pracctices to for the detection of SARS-CoV-2 virus?Describe two techniques that can be used to purify viruses and discuss the advantages and disadvantages of these methodsWrite a lab report on enumeration of lytic viruses : the plaque assay including the sections: introduction, Materials and Methods, Results and Discussion, and citing peer reviewed sources use this data for results section Group Number Number of Plaques Dilution Factor of Plate Original Concentration of Phage 1 58 10^3 5.80E+04 2 37 10^3 3.70E+04 3 44 10^3 4.40E+04 4 63 10^3 6.30E+04 5 36 10^2 3.60E+03 6 65 10^3 6.50E+04 7 260 10^2 2.60E+04 8 39 10^3 3.90E+04 9 178 10^2 1.78E+04 10 192 10^2 1.92E+04 11 274 10^2 2.74E+04 12 224 10^2 2.24E+04 Class Average 3.52E+04
- What is the importance of the agar overlay technique in virus isolation?A 42 year old man developed a “flu-like” syndrome with fever, sore throat, headache, and myalgia. Viral culture was ordered for laboratory confirmation of influenza. Which of the following would be the best specimen for isolating virus responsible for the infection? A- Blood B- Nasopharyngeal washing C- Stool D- Saliva E- Vesicle fluidDiscuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods: Restriction Fragment Length Polymorphism