describe how the exercise of streaking a loop full of lytic T4-phage suspension over a petri dish with E. coli B, Enterobacter aerogenes and Staphylococcus aureus demonstrates the principle of phage typing.
Find answers to questions asked by students like you.
Q: Discuss retroregulation and its significance in the biology of bacteriophage lambda during: a)…
A: Introduction Retroregulation can be defined as the control of gene expression from sites distal to…
Q: Describe the growth pathway the bacteriophage lambda will take when used to infect a mid-log-phase…
A: A bacteriophage is a virus that causes bacteria to become infected. Since bacteriophages consume…
Q: Describe in detail the kinetics of phage infection followed by host lysis in bacteria. with…
A: Bacteriophages are viruses that infect bacteria. The term "bacteriophage" literally means "bacteria…
Q: In generalized transduction.... Choose from the answers below: random fragments of degraded…
A: Generalized Transduction : It occurs when random pieces of bacterial DNA are packaged into the phage…
Q: Ahmed had isolated bacteria from the sputum of a patient that seemed somewhat resistant to their…
A: Click to see the answer
Q: Ahmed had isolated bacteria from the sputum of a patient that seemed somewhat resistant to their…
A: The phage-resistant bacteria may develop with time just like the antibiotic-resistant bacteria.…
Q: A conjugation-deficient strain of A. radiobacter is used to combat crown gall disease. Explain how…
A: Disease: When an organism’s body system does not work properly or any organ affects by viruses,…
Q: In a mixed infection assay between h+r+ and hr phage, where h and r are two genes that govern plaque…
A: A viral plaque is a recognisable structure that forms when a viral sample is introduced to a cell…
Q: Compare the effect on the host cell of a lytic (virulent)phage and a lysogenic (temperate) phage.
A: Virus are obligate parasites. Viruses either contains DNA or RNA as genetic material and a protein…
Q: Discuss some advantages and disadvantages of bacteriophage therapy in treating bacterial infections.
A: Phage therapy (PT) is referred to as bacteriophage therapy. It generally uses viruses to treat…
Q: Describe in general terms how bacteriophage lambda regulates the switch between lytic and lysogenic…
A: Bacteriophages are very small viral particles that infect bacterial host cells. The replication of…
Q: what is the explanation for bacteriophage specificity when it comes to infecting specific bacterium
A: A bacteriophage is a virus that infects bacteria and archaea and replicates within them. They are…
Q: Phage M13 has often been used for Select one: a. phage display b. generalized transduction O c.…
A: Virus that infects bacteria are called phages. M13 is a filamentous phage consisting of ss DNA. They…
Q: Describe any three vectors less method of introducing the rDNA into a competent host cell?
A: rDNA is the recombinant DNA which is formed by combining desired genes from different organisms. It…
Q: What is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic…
A: E. coli is a gram-negative bacillus and some of its strains have evolved into pathogens. One of…
Q: What is the purpose of including a tube in the phage assay in which only the E. coli culture is…
A: Viruses are minute particles that are made up of only their genetic material which may be DNA or RNA…
Q: What is the purpose of including a tube in the phage assay in which only the E. coli culture is…
A: Insects that feed on fecal, dung material and raw sewage are termed as coprophagous insects. These…
Q: Compare and contrast the lysogenic and lytic cycles of phage λ.
A: Phage lambda is a bacteriophage which infects E.coli bacteria. Bacteriophages are the viruses that…
Q: when various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid…
A: Bacteriophages are the viruses that infect and replicate within the bacterial host. They are of two…
Q: Briefly describe the differences between the lytic cycle of virulent phages and the lysogenic cycle…
A: Bacteriophages (phages) are obligate intracellular viruses that specifically infect bacteria. The…
Q: The figure provided portrays the integration of Lambda phage into a host chromosome at the att site,…
A: In order to determine the change of the host bacterium status from gal- to gal +, the galactose…
Q: Discuss some advantages and disadvantages of bacteriophage therapyin treating bacterial infections.
A: Bacteriophage is defined as the type of virus that infects bacteria. It is also called as bacterial…
Q: Describe how a plaque assay is used to detect and estimate the numbers of phage particles.
A: Plaque is a term used in microbiology for a clear zone on a bacterial culture plate that indicates…
Q: solated bacteria from the sputum of a patient that seemed somewhat resistant to their antibiotic ce…
A: Mycobacterium tuberculosis is a bacteria which attacks the lungs and cause the disease tuberculosis.…
Q: In 1944, Avery, Macleod, and McCarty provided strong evidence that DNA is the hereditary material in…
A: For this they started through a series of biochemical steps and purified the transforming principle…
Q: Why would a lab do phage typing rather than molecular typing?
A: In laboratories, various microorganisms including bacteria of a single strain or multiple strains…
Q: You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A…
A: Bacteriophage : these are the viruses that attack on barterial cells and use them to produce more…
Q: If you Infect the Jurkat T lymphocyte cell line with HIV-1 strain IIIB. Lyse the infected cells at…
A: A virus is a microscopic infectious agent that could infect and live only within the living…
Q: The First rDNA experiment was in lambda phage fragment incorporated into SV40 vector.
A: The first custom-made creature having recombined or "recombinant" DNA was created in Stanley Cohen…
Q: You have constructed a cDNA library in a phagemid vector. Describe how you would screen the library…
A: Phagemid vectors are plasmid DNA [Deoxyribonucleic acid] containing a small portion of the genome of…
Q: A great concern in hospitals is the spread of antibiotic resistance genes among bacteria to generate…
A: Antibiotic resistance: when the bacteria survive in the presence of target antibiotics that are…
Q: Suppose you transformed a plasmid vector carrying a human interferon gene into E. coli but none of…
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: Give three reasons why Salmonella strains are very useful for mutagenesis studies
A: Ans- The standard set of bacterial strains that are used in the mutagenicity testing of…
Q: Why is phage conversion considered beneficial to host cells?
A: Phages are viruses that have bacterial cells as their host.
Q: When various strains of λ phage are seeded on a lawn of E. coli, they can form clear or turbid…
A: The Operon is the regulatory system present in DNA in cluster form, which contains structural genes…
Q: CRISPR has been well and truly deployed in the fight against COVID-19, but what potential does it…
A: It is a powerful technology for editing gene functions, alter the DNA sequences, and modify the…
Q: You are given two strains of E. coli. The Hfr strain is arg+ ala+ glu+ pro+ leu+ T s; the F− strain…
A: F- Strain susceptible to Pen but ltfr strain resistant So, ltfr strain must be the time it has per r…
Q: In recombination studies of the rII locus in phage T4, what is the significance of the value…
A: Seymour Benzer developed an experimental system around 1950s for analyzing various subparts of a…
Q: There is some recent research on the use of bacteriophages to inhibit pathogenic bacteria as…
A: Antibiotics are the drugs used to inhibit or kill bacteria.
Q: What are the advantages and disadvantages to bacteriophages of the lytic and lysogenic rep
A: Bacteriophages : A type is a type of virus that infects bacteria . There are two types of…
Q: What is required to package the T4 genome into its phage head?
A: Introduction T4 is a type bacteriophage which infects the E coli bacteria (Escherichia coli). It is…
Q: During the late stage T4 phage infection, the phage DNA is synthesized by Select one: O a. the DNA…
A: Bacteriophages are viruses that have bacterial as host cells.
Q: Discuss the lysogrnic induction of bacteriophage lambda in coli cells
A: Viruses are microbes that have both living and non-living characteristics. They are obligate…
Q: You have isolated a beta-lactamase producing Staphylococcus aureus (not a MRSA strain) from an…
A: It's a microbiology subject question, in it, they have isolated a bacteria called name…
Q: n Hershey and Chase’s experiment investigating which biological molecule carries genetic…
A: Hershey and Chase studied cells of the intestinal bacteria ie E.coli after injection by virus T2.
Q: What role did the transposon play in the transfer of resistance?
A: Click to see the answer
Q: Does the plasmid integrate into host genome when the AAV is introduced to patient? Explain
A: Plasmid known as an autonomously, circular and self-replicating molecules whose vectors have been…
Q: Name two stages in a lytic infection where lysozyme is used. Describe the role of lysozyme during…
A: The term lytic infection is related to viruses. A virus is a very small submicroscopic agent that is…
Q: Draw a diagram of the one-step growth curve ofvirus replication, and briefly explain why the…
A: The bacteria and viruses are totally different from each other. They are different from each other…
Q: SV40 vectors are derived from Which of the following term is appropriate for the blank? Lütfen…
A: The SV40 vector is a replacement vector. It can be used as an early or late replacement vector by…