Question
Asked Nov 4, 2019
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describe how the exercise of streaking a loop full of lytic T4-phage suspension over a petri dish with E. coli B, Enterobacter aerogenes and Staphylococcus aureus demonstrates the principle of phage typing.

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Expert Answer

Step 1

Bacteriophages are viruses that specifically infect bacteria; each bacteriophage has a specific bacterial host.  Phage typing is vastly used to detect single strains of bacteria; it is majorly employed in tracing the source of bacterial disease outbreaks. On streaking a loop full of T4 lytic phage over a petri dish streaked with E.coli, Enterobacter aerogenes and Staphylococcus aureus followed by incubation under favorable conditions leads to the formation of plaques. On further observation plaque forming units (PFU) can be analyzed to confirm which bacterial species have been lysed.

Step 2

This exercise enables in demonstrating the principle of phage typing. The T4 lytic phage is also referred to as the Escherichia virus T4 and the specific host of the T4 lytic phage is Escherichia coli. When a loop full of lytic T4 phage suspension is streaked over a petri dish with streaked colonies of E.coli, Enterobacter aer...

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