c only Eco01091 20/4Plai 48PstAPI 179Aath 2613Liet 183Sspl 2501Ehel, SspDI 235Pdmi 2234Bogl 2215Scal 2177Tsol 21082456MCSBsaX 659pUC18/192686 bpNmeAlli 1822Afl, Pscl 806Gsul 1/84Ctri0l 17/19,Eco311 1/66Eam1105! I691466rep (PMB1)Cail 1217Psp-i 1110Figure 1(https://www.fishersci.com/shop/products/thermo-scientific-puc18-pue19-dna-50-g/fersd0051)Figure I above is showing the plasmid map of PUC18. Discuss how screening is carried outif this plasmid is used for cloning.Explain the THREF. (3) important regions of this plasmid that enable it to work efficiently asa cloning vector?b)State the differences between the plasmid in Figure i with PBR322 in terms of theirsercening procedures.c)Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid inFigure 1.d)bla (Ap)

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Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter46: Ecosystems

Section: Chapter Questions

Problem 24RQ: Which of the following approaches would be the most effective way to reduce greenhouse carbon...

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State the differences between the plasmid in Figure 1 with pBR322 in terms of their sercening procedures. c)