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Describe how the SCAM analysis was performed so that it maintains replicatability.

Describe how the SCAM analysis was performed so that it maintains replicatability.

Anatomy & Physiology
Anatomy & Physiology
1st Edition
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
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Describe how the SCAM analysis was performed so that it maintains replicatability.

kDa 55 Cysless 35 20 10 F16C E39C L860 G125C + + +U M18C V51C Y96C F139C M36C K72C ---+ MTSES + - NEM + + + MPEG5K |D106C +P ←U R143C +P ←U + MTSES - NEM + MPEG5K +P +U + MTSES - NEM +++ MPEG5K +P ←U + MTSES NEM +++ MPEG5K Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell lysates from cells harbouring plasmids expressing cysteine-free ('Cysless') or single cysteine variants of alaE with and without treatment with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein are indicated with arrows.
Transcribed Image Text:kDa 55 Cysless 35 20 10 F16C E39C L860 G125C + + +U M18C V51C Y96C F139C M36C K72C ---+ MTSES + - NEM + + + MPEG5K |D106C +P ←U R143C +P ←U + MTSES - NEM + MPEG5K +P +U + MTSES - NEM +++ MPEG5K +P ←U + MTSES NEM +++ MPEG5K Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell lysates from cells harbouring plasmids expressing cysteine-free ('Cysless') or single cysteine variants of alaE with and without treatment with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein are indicated with arrows.
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