Determination of Creatinine in Serum and Urine Experiment; Question: Why are using this materials/chemicals in this experiment? *10% sodium tungstate *0.75 M NaOH Creatinine depot standard *0.67 N H2SO4 *Picric acid (0.04 M) Procedure: 3 mL of water, 1 mL of 10% sodium tungstate, 2 mL of 0.67 N H2SO4 are added onto the serum (2 mL), mixed well, and filtered after 5-10 minutes (protein-free serum filtrate). 3 mL of this filtrate is used for the test. All tubes are mixed, incubated at room temperature for 20 minutes and read against the reagent blank at 520 nm. First, the serum volume in the test tube is calculated. Since working with 3 mL standard, the absorbance of the standard tube belongs to 30 µg creatinine.

Determination of Creatinine in Serum and Urine Experiment; Question: Why are using this materials/chemicals in this experiment? 10% sodium tungstate 0.75 M NaOH Creatinine depot standard 0.67 N H2SO4 Picric acid (0.04 M) Procedure: 3 mL of water, 1 mL of 10% sodium tungstate, 2 mL of 0.67 N H2SO4 are added onto the serum (2 mL), mixed well, and filtered after 5-10 minutes (protein-free serum filtrate). 3 mL of this filtrate is used for the test. All tubes are mixed, incubated at room temperature for 20 minutes and read against the reagent blank at 520 nm. First, the serum volume in the test tube is calculated. Since working with 3 mL standard, the absorbance of the standard tube belongs to 30 µg creatinine.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 7.2P

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