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ACTIVITY 5.4.1 Synthesis of a Protein: A Simulation Activity In this activity, you will be provided with the DNA nucleotide sequence that codes for a hypothetical protein. The code will be provided to you in three fragments. You will have to tran- scribe the code into mRNA, remove an intron segment, and translate the mRNA into the protein. In addition, you will have to identify the beginning fragment, the middle fragment, and the end fragment. Procedure 1. Copy each of the following quences onto a separate piece of paper. Sequence A TCTTCCCTCCTAAACGTTCAACCGGTTCTTAATCCGC CGCCAGGGCCCCGCCCCTCAGAAGTTGGT Sequence B TCAGACGTTTTTGCCCCGTAACAACTTGTTACAA CATGGTCATAAACGTCAGAGATGGTCAATCTCTTAAT GACT Sequence C TACAAACATGTAAACACACCCTCAGTGGACCAACTC CGCA ACATAAACCAAACACCGCTCGCGCCGAAAAA GATATGG 2. Divide the sequences into triplets (codons) by putting a slash between each group of three bases. ACTIVITY 5.4.1 continued 3. Transcribe the DNA into mRNA. 4. Identify the middle, end, and beginning sequence. Use your knowledge of start and stop codons to help you figure it out. 5. Remove codons 24 to 66, including codon 66. 6. Translate the mRNA into protein using the genetic code. Analysis (a) Which fragment was the beginning fragment? How do you know? >) Which fragment was the end fragment? How do you know? c) Codons 24 to 66 represent an intron. At what point in the process of protein synthesis are introns removed? What is the name of the enzyme responsible for this excision? (d) How many amino acids does this protein contain? (e) Is this genetic sequence eukaryotic or prokaryotic? How do you know? (f) If you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not? (g) Provide the anticodon sequence that would build this protein.

ACTIVITY 5.4.1 Synthesis of a Protein: A Simulation Activity In this activity, you will be provided with the DNA nucleotide sequence that codes for a hypothetical protein. The code will be provided to you in three fragments. You will have to tran- scribe the code into mRNA, remove an intron segment, and translate the mRNA into the protein. In addition, you will have to identify the beginning fragment, the middle fragment, and the end fragment. Procedure 1. Copy each of the following quences onto a separate piece of paper. Sequence A TCTTCCCTCCTAAACGTTCAACCGGTTCTTAATCCGC CGCCAGGGCCCCGCCCCTCAGAAGTTGGT Sequence B TCAGACGTTTTTGCCCCGTAACAACTTGTTACAA CATGGTCATAAACGTCAGAGATGGTCAATCTCTTAAT GACT Sequence C TACAAACATGTAAACACACCCTCAGTGGACCAACTC CGCA ACATAAACCAAACACCGCTCGCGCCGAAAAA GATATGG 2. Divide the sequences into triplets (codons) by putting a slash between each group of three bases. ACTIVITY 5.4.1 continued 3. Transcribe the DNA into mRNA. 4. Identify the middle, end, and beginning sequence. Use your knowledge of start and stop codons to help you figure it out. 5. Remove codons 24 to 66, including codon 66. 6. Translate the mRNA into protein using the genetic code. Analysis (a) Which fragment was the beginning fragment? How do you know? >) Which fragment was the end fragment? How do you know? c) Codons 24 to 66 represent an intron. At what point in the process of protein synthesis are introns removed? What is the name of the enzyme responsible for this excision? (d) How many amino acids does this protein contain? (e) Is this genetic sequence eukaryotic or prokaryotic? How do you know? (f) If you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not? (g) Provide the anticodon sequence that would build this protein.

Biochemistry
Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter3: Amino Acids And Peptides
Section: Chapter Questions
Problem 54RE: THOUGHT QUESTION Imagine we identify a gene that is directly responsible for the effects of...
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ACTIVITY 5.4.1 Synthesis of a Protein: A Simulation Activity In this activity, you will be provided with the DNA nucleotide sequence that codes for a hypothetical protein. The code will be provided to you in three fragments. You will have to tran- scribe the code into mRNA, remove an intron segment, and translate the mRNA into the protein. In addition, you will have to identify the beginning fragment, the middle fragment, and the end fragment. Procedure 1. Copy each of the following quences onto a separate piece of paper. Sequence A TCTTCCCTCCTAAACGTTCAACCGGTTCTTAATCCGC CGCCAGGGCCCCGCCCCTCAGAAGTTGGT Sequence B TCAGACGTTTTTGCCCCGTAACAACTTGTTACAA CATGGTCATAAACGTCAGAGATGGTCAATCTCTTAAT GACT Sequence C TACAAACATGTAAACACACCCTCAGTGGACCAACTC CGCA ACATAAACCAAACACCGCTCGCGCCGAAAAA GATATGG 2. Divide the sequences into triplets (codons) by putting a slash between each group of three bases. ACTIVITY 5.4.1 continued 3. Transcribe the DNA into mRNA. 4. Identify the middle, end, and beginning sequence. Use your knowledge of start and stop codons to help you figure it out. 5. Remove codons 24 to 66, including codon 66. 6. Translate the mRNA into protein using the genetic code. Analysis (a) Which fragment was the beginning fragment? How do you know? >) Which fragment was the end fragment? How do you know? c) Codons 24 to 66 represent an intron. At what point in the process of protein synthesis are introns removed? What is the name of the enzyme responsible for this excision? (d) How many amino acids does this protein contain? (e) Is this genetic sequence eukaryotic or prokaryotic? How do you know? (f) If you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not? (g) Provide the anticodon sequence that would build this protein.
Transcribed Image Text:ACTIVITY 5.4.1 Synthesis of a Protein: A Simulation Activity In this activity, you will be provided with the DNA nucleotide sequence that codes for a hypothetical protein. The code will be provided to you in three fragments. You will have to tran- scribe the code into mRNA, remove an intron segment, and translate the mRNA into the protein. In addition, you will have to identify the beginning fragment, the middle fragment, and the end fragment. Procedure 1. Copy each of the following quences onto a separate piece of paper. Sequence A TCTTCCCTCCTAAACGTTCAACCGGTTCTTAATCCGC CGCCAGGGCCCCGCCCCTCAGAAGTTGGT Sequence B TCAGACGTTTTTGCCCCGTAACAACTTGTTACAA CATGGTCATAAACGTCAGAGATGGTCAATCTCTTAAT GACT Sequence C TACAAACATGTAAACACACCCTCAGTGGACCAACTC CGCA ACATAAACCAAACACCGCTCGCGCCGAAAAA GATATGG 2. Divide the sequences into triplets (codons) by putting a slash between each group of three bases. ACTIVITY 5.4.1 continued 3. Transcribe the DNA into mRNA. 4. Identify the middle, end, and beginning sequence. Use your knowledge of start and stop codons to help you figure it out. 5. Remove codons 24 to 66, including codon 66. 6. Translate the mRNA into protein using the genetic code. Analysis (a) Which fragment was the beginning fragment? How do you know? >) Which fragment was the end fragment? How do you know? c) Codons 24 to 66 represent an intron. At what point in the process of protein synthesis are introns removed? What is the name of the enzyme responsible for this excision? (d) How many amino acids does this protein contain? (e) Is this genetic sequence eukaryotic or prokaryotic? How do you know? (f) If you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not? (g) Provide the anticodon sequence that would build this protein.
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