Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer. What factor(s) did you take into consideration when selecting the number of passes? Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative? What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a? If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend?
Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer. What factor(s) did you take into consideration when selecting the number of passes? Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative? What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a? If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend?
Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter21: Musculoskeletal And Anti-inflammatory Drugs
Section: Chapter Questions
Problem 15CRQ
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- Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer.
- What factor(s) did you take into consideration when selecting the number of passes?
- Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative?
- What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a?
- If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend?
Transcribed Image Text:1. Bacillis brevis are lysed using a valve-type homogenizer. The extent of disruption
depends on the applied pressure and the number of passes through the homogenizer
chamber and can be described by the equation, In(1 - R) = -kN pa
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