Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the experiment you did in thin layer chromatography of plant pigments? How do you say so?
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- why steroids do not show luminescence on thin layer chromatographic plates?In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.
- The following strip of chromatography paper has been placed in a polar solvent Which pigments the most polar?what does it mean if a pigment (let' say 'yellow') traveled beyond the solvent front in paper chromatography?In paper chromatography of spinach leaves: (a) Which of the pigments moves faster and why? (b) Which of the pigments stands out?
- What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).The two most abundant pigments in the chromatography results were carotene and Xanthophyll. What colors are absorbed or reflected by various pigments and how much is there?briefly explain the principle behind this technique; affinity chromatography using a diagram to illustrate.
