Discuss what is meant by the Oxygen effect, the oxygen enhancement ratio and some possible mechanisms. In your discussion, research and explain then Howard Flanders and Alper analysis.
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- How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.Write the procedure for the following 1. Celloidin embedding 2. Vacuum embedding 3. Epoxy embedding 4. resin embeddingDraw and label detailed apparatus of the steam distillation apparatus used for the Isolation of Eugenol experiment.
- What were the results from the FTM (Fluid Thioglycerate Media) for the three bacteria (M. luteus, C. sporogenes, S. aureus)? What were the oxygen classification given to each? Please explain what this classification means.If you conducted a Rhizobium isolation and it was a succesful, will you have proven that it fixes nitrogen? Please give details on how you would know. What experiment could you do to demonstrate this property? Thank youFlame Test (Unknown) View the Unknowns to see the results of the flame test. Record Your Observations (clearly and neat hand writing please)
- In etanol determination by specrophotometry, explain the purpose of the negative controls included in a protocol, what do they control for? (What will you learn if the negative control gives an unexpected result. i.e. returns a positive result?)Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.2061) give detail note ( giving points ) on three generation of photosensitizer (PSS) with example and also highlight the role of third generation photosensitizer . [ please refer research paper to give answer at end end of the answer please write the DOI number ]