Discuss TWO (2) isotope-based methods useful in microbial community analysis. In your answer, outline at least TWO (2) advantages and TWO (2) limitations associated with each method

THIS IS A MULTIPART QUESTION, PLEASE ANSWER ALL Using the image: 1. How would you categorize this medium? (Living/nonliving, Synthetic/complex, Rich/minimal) Briefly explain your responses. 2. Name the ingredient(s) that match the following nutrients: a. Source(s) of carbon b. Source(s) of nitrogen c. At least one source of minerals d. At least one source of growth factors, if any 3. What about this medium would change if agar were added as an ingredient? 4. Would you expect this medium to be differential, selective, or both? Explain your response.

Suppose your town was interested in building a chemical waste storage facility in your neighborhood. Consider “lessons learned” from past failures of ways storage, dumping, environmental contamination, and successful applications of bioremediation. What are the priorities town officials should consider before building the site? What possible concerns are raised if the site were to be used?