Based your understanding of enzyme kinetics and inhibitors which line is the assay with the inhibitor? top line on the right side of the graph bottom line on the right side of the graph b) Determine the scale on the x and y axes and provide the coordinates for the point corresponding to 33 nM substrate on the without inhibitor curve. X: Y: c) What type of inhibition could be demonstrate by this graph? (select all that apply) competitive
a) Based your understanding of enzyme kinetics and inhibitors which line is the assay with the inhibitor?
- top line on the right side of the graph
- bottom line on the right side of the graph
b) Determine the scale on the x and y axes and provide the coordinates for the point corresponding to 33 nM substrate on the without inhibitor curve.
X:
Y:
c) What type of inhibition could be demonstrate by this graph? (select all that apply)
- competitive
- uncompetitive
- Mixed
- non- competitive
- feedback inhibition
- product inhibition
d) What does that tell you about how the inhibitor interacts with the enzyme? (what form does it bind to and where)(2 sentences MAX)
e) Draw the Michaelis-Menten graph you would predict for the enzyme using 50 nM and 100 nM of enzyme in your assays. (this can be hand drawn, it does not have to be excel generated.)
![You assay the activity of 50 nM of your favorite enzyme in the presence and absence of an inhibitor.
Below is the data for some of the substrate concentrations you assayed without the inhibitor, along
with the Lineweaver-Burke plot of the data both with and without the inhibitor.
[substrate] (nM)
20
25
50
100
200
Initial Velocity
(s/uM)
μM/s of product
produced without Inhibitor
1.067
1.250
1.905
2.581
3.137
0
1/[substrate] (1/nM)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1d39cd43-eb6b-44d1-81e0-17a716330bd6%2Feddc8a3f-c57d-41ad-ac1a-0a4f62f43a5d%2Fr4epqff_processed.png&w=3840&q=75)
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Draw the Michaelis-Menten graph you would predict for the enzyme using 50 nM and 100 nM of enzyme in your assays.
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