Drug substances are useful based on their biological activity. Thus, an assay must be developed to ensure that this important function is preserved during purification. There are three major types of biological activity assays including animal model assays, cell-line-derived assays, and in vitro biochemical assays.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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  1. Drug substances are useful based on their biological activity. Thus, an assay must be developed to ensure that this important function is preserved during purification. There are three major types of biological activity assays including animal model assays, cell-line-derived assays, and in vitro biochemical assays. Please find an example of each (in literature, online, etc.) and briefly Include a reference for each (no particular reference format required, can simply include a web link if easiest).
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Introduction 

Drug substances, also known as active pharmaceutical ingredients (APIs), are the chemical compounds or molecules that are used to produce medications or drugs. They are the components in a drug that have a therapeutic effect on the body. When used in appropriate doses, these drug substances are intended to prevent, cure, or treat diseases or medical conditions. 

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