Explain CRISPR screening. Explain the rationale of using it and the objectives that can be achieved in detail. Explain how it is done.
Q: Explain CRISPR screening in detail. Explain the rationale of using it and the objectives that can be…
A: Genetic screening is referred to as the process used to test the population for a genetic disorder…
Q: Explain how DNA microarrays are used in molecular profiling ofcancerous tumors.
A: DNA microarrays are microscopic slides that are printed with thousands of tiny spots in defined…
Q: Discuss the E-test of antibiotic testing. pls make it clear, concise and organize
A: Antibiotic testing is done to assess the usage of various doses of antibiotics for different…
Q: Explain the difference between in vivo and ex vivogene therapy.
A: It involves the supplementation of a defective gene with a functional allele. It is achieved by gene…
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A: Liquid chromatography (LC) and the targeted attachment of antibodies or related substances are both…
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A: Anchorage-independent growth, which is a hallmark of carcinogenesis, is the capacity of transformed…
Q: Write five applications of CRISPR/Cas system in diagnosis and therapy with examples
A: CRISPR-Cas9 is genome editing tool that enables geneticists medical researchers to edit parts of the…
Q: Define personalized medicine and molecular profiling.
A: Personalized medicine is an innovation towards enhancing patient care system. This technique relies…
Q: Describe the benefits of using whole genome sequencing for diagnosis.
A: The whole genome shotgun method involves cloning, followed by accurate computer sequencing of the…
Q: Describe what CRISPR is and explain the overall process of how it works. Identify and discuss 3-4…
A: CRISPR : It stands for Clustered Regularly Interspaced Short Palindromic Repeats Repetitive DNA…
Q: Explain the steps to transform a plasmid carrying a gene of interest into a competent host cell…
A: The deliberate modification of an organism's genetic information by directly changing its nucleic…
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A: Immunoassay: This is basically a biochemical analytical technique that help detecting and measuring…
Q: How are microarrays used to analyse the response of cells in drug therapy
A: Microarrays or DNA microarrays is a molecular technique used in biological laboratories where we can…
Q: Explain the basic principles of Elispot Assay
A: ELISPOT assay is an Enzyme-Linked Immune adsorbent Spot assay that is used for testing cellular…
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A: It is very difficult to identify the slow-growing bacteria using conventional culturing methods. Use…
Q: Explain the basic methods behind the Western blot andcomplement fixation tests.
A: Western blot is a specific test that is used to detect the presence of a target protein in the given…
Q: Discuss an example of ex vivo and of in vivo genetherapy.
A: Gene therapy is the science that deals with the insertion of a gene into the defective cells having…
Q: With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular…
A: INTRODUCTION It is a real-time polymerase chain reaction also known as qPCR is a Molecular…
Q: State three clinical uses of DNA-based tests.
A: Genetic testing is also known as DNA testing which provides details about the person's genes and…
Q: ELISA link____ to___ in order to create a colorimetric assay
A: Antigens and antibodies react specifically to form the Antigen-Antibody complex. Based on this…
Q: Define the therapeutic gene?
A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…
Q: Explain the process of “blue-white” screening ?
A: Blue-white screening is a technique which allows proper detection of recombinant bacteria for…
Q: State and describe the benefits of using whole-genome sequencing for diagnosis.
A: The whole genome shotgun method involves cloning, followed by accurate computer sequencing of the…
Q: Describe the limitations of RF Agglutination test
A: Note: Since you have asked multiple question, we will solve the first question for you. If you want…
Q: PCR is a useful tool for early diagnosis of an infectious disease.Elaborate.
A: A disease refers to an abnormal condition in the body that have negative impact on the function or…
Q: Explain how in vivo testing differs from in vitro testing.
A: In vitro and In vivo are the methods used in scientific studies for the research. The various…
Q: efine gene therapy in detail.
A: Gene therapy is a process that includes inserting a normally functioning gene into a cell to replace…
Q: Discuss the Genetically Modified Organism (Benefits and Risks)
A: GMOs are organisms whose genetic material have been altered through the application of genetic…
Q: a. Mention the procedure of doing blood group by direct method (Forward typing) b. How will you…
A: Blood is a circulating fluid in our body that is composed of red blood cells, white blood cells and…
Q: Compare and contrast ex vivo and in vivo gene therapy as approaches for delivering therapeutic…
A: Viral vectors are used as carriers for normal genes that replace the defective genes within the…
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A: There are several genetic testing methodologies that have been employed in identification of…
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A: Ans- The standard set of bacterial strains that are used in the mutagenicity testing of…
Q: Explain CRISPR screening in detail. Explain the rationale of using it and the objectives that can be…
A: Clustered regularly interspaced short palindromic repeats (CRISPR) is used as a gene-editing tool.…
Q: Briefly discuss the process of rapid versus slow digitalization as well as the use of the antidote…
A: Digoxin immune fab is a drug that is used to treat and control digoxin toxicity. Patients with a…
Q: Explain the ELISA protocol and the purpose of each step
A: ELISA stands for Enzyme-Linked-Immunosorbent Assay.
Q: How can genetic testing help in cancer diagnosis? Briefly explain
A: Cancer is a group of disease which involves abnormal growth of cell with the ability to spread to…
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A: Introduction :- Antimicrobial chemotherapy is used to treat infectious diseases by fighting the…
Q: Explain the rationale and achievable goals behind using a genetic screen(like Heidelberg screen).…
A: Genetic screening such as Heidelberg screening is also called mutagenesis screening. This technique…
Q: Briefly describe how toe Bradford protein assay works
A: Introduction There are several methods to determine various biomolecules either quantitatively or…
Q: Explain the types of sequencing techniques
A: DNA sequencing techniques are methods used for the identification of order or sequence of…
Q: What is high throughput screening in the discovery of drug ?
A: Drug discovery is a highly complex and multidisciplinary process which goal is to identify new…
Q: How is DNA microarray used in detecting cancer?
A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…
Q: Define the advantage of using PCR for infectious disease diagnosis.
A: Polymerase Chain Reaction is referred to as PCR. The process of amplifying DNA sequences and…
Q: ecombinant DNA technology has provided a broad range of tools to help physicians in the diagnosis of…
A: Every pathogen has some unique sequence in their DNA that can be targeted by using probes. Thus…
Q: What is the difference between the total and faecal coliform
A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…
Q: Discuss the applications of molecular diagnostic tests
A: Introduction :- Molecular diagnostic tests refers to the diagnosis of a disease at the molecular…
Q: Discuss briefy how a probe is used in molecular diagnostics.
A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…
Explain CRISPR screening. Explain the rationale of using it and the objectives that can be achieved in detail. Explain how it is done.
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- Explain the advantages of using cell based assays for drug screening? Pls no chat gptState and describe the benefits of using whole-genome sequencing for diagnosis.Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? Explain
- Discuss an example of ex vivo and of in vivo genetherapy.describe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.Write down basic principle of ELISA and its main steps? Write names of different types of ELISA. (Subject: Biotechnology)
- When it comes to establish a biotechnology laboratory, certain equipment is necessary to be present in the lab and also, it is crucial to follow certain dos and don’ts to ensure safety and hygiene. What specific equipment is essential for establishing a biotechnology laboratory? Also, specify some rules that must be followed while working in biotechnology laboratories?What is probe? Why it is used in the library screening ?explain why gene mapping is important and how this technique can be used in disease investigation?