Q: Explain CRISPR screening in detail. Explain the rationale of using it and the objectives that can be…
A: Genetic screening is referred to as the process used to test the population for a genetic disorder…
Q: Explain how DNA microarrays are used in molecular profiling ofcancerous tumors.
A: DNA microarrays are microscopic slides that are printed with thousands of tiny spots in defined…
Q: Discuss the E-test of antibiotic testing. pls make it clear, concise and organize
A: Antibiotic testing is done to assess the usage of various doses of antibiotics for different…
Q: Cystic fibrosis (CF) test to screen for mutations associated with the CFTR gene. Positive samples…
A: Cystic fibrosis is a condition that affects the glands that generate sweat, mucus, and intestinal…
Q: ii) In order to create a western blot, SDS-PAGE is first performed to separate the proteins. After…
A: SDS-PAGE is a protein separation procedure, most widely used in immunoblotting. It can be done once…
Q: Involves comparing the genomes Genomics Metabolomics Proteomics all of these
A: ▪︎Genomics is the study of full genetic complement of the organism or it is the study of all the…
Q: Experiment: Bradford protein assay give the answers (4-5 lines) of review questions in the end. the…
A: The proteins are formed by the polymerization of amino acids. Thus the amino acids are proteins that…
Q: Tm value, the GC-content of the selected DNA sequence and the absolute nucleotide composition of the…
A: DNA stands for deoxyribonucleic acid and it consists of 4 types of nitrogen bases, adenine, guanine,…
Q: briefly describe the working principle of both direct and indirect assays of biosensor taking…
A: Biosensors are defined as an analytical device that has a physicochemical and biological components.…
Q: Explain CRISPR screening. Explain the rationale of using it and the objectives that can be achieved…
A: Genetic screening is referred to as the process used to test the population for a genetic disorder…
Q: Explain the steps of preparing a cell culture stock from a T25 flask.
A: Cell culture is the specialized process used in molecular biology to remove cells from plants and/or…
Q: Differential protein expression of a protein A in two protein samples is studied by gel…
A: Differential expression analysis is the process by which a quantitative changes is the expression of…
Q: SDSPAGE is similar to other protein detection and quantification methods such as Native PAGE,…
A: Sodium dodecyl polyacrylamide gel electrophoresis or SDS PAGE refers to the technique of separating…
Q: PET relies on radioactive substances administeredseveral minutes before the scan. How is PET used in…
A: Positron emission tomography (PET) is a medical imaging technique that reveals the functioning of…
Q: 8-12 years old child's eye retina area is yellow. Which methodology do you follow to develop a…
A: Our eyes appears as orange due to the presence of a layer of pigmented cells inside the retina. This…
Q: Fluorescence confocal microscopy (FCM) - STK38 monoclonal antibody (M01), clone 2G8- 1F3. 200 μm a.…
A: The STK38 Monoclonal Antibody from LifeSpan BioSciences is a Mouse Monoclonal antibody. This…
Q: A) One assay format that might be appropriate for developing a point of care diagnostic kit to…
A: INTRODUCTION A) Diagnostic virology is rapidly getting into the mainstream of clinical medicine as a…
Q: State and describe the benefits of using whole-genome sequencing for diagnosis.
A: The whole genome shotgun method involves cloning, followed by accurate computer sequencing of the…
Q: Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing
A: In this question, we have to give out line the step of extraction of DNA molecules.
Q: Why is an antibody used in this experiment?
A: Chromatin Immunoprecipitation (ChIP) is a type of technique based on immunoprecipitation and is…
Q: Which statement below about Bradford assay is wrong? O Coomassie Brilliant Blue dye is used to bind…
A: You have asked 2 questions. I will answer 1st question, as per guidelines. The Bradford assay is…
Q: 3. Primer Design You are analyzing the
A: Primer is a short stretch of oligonucleotide which is designed to copy the gene sequence of interest…
Q: Describe the main technique for amplifying a segment of DNA (like the one you suspect is involved in…
A: Polymerase chain reaction is a method used to make several copies of a specific target DNA.
Q: Ten example of Human protein in therapeutic genetic technology that have available in genetic…
A: Genetic Engineering: Genetic engineering is the process of altering an organism's genetic makeup…
Q: Explain CRISPR screening in detail. Explain the rationale of using it and the objectives that can be…
A: Clustered regularly interspaced short palindromic repeats (CRISPR) is used as a gene-editing tool.…
Q: Advantages and disadvantages of using computer simulations (ex. protein database) over tangible…
A: Thank you for the question Answer :- Computer simulation is predicting the outcome of a real world…
Q: a. Briefly discuss (using three sentences) how the concepts and/or techniques in molecular biology…
A: As COVID-19 was discovered in Hubei Province, China, in December 2019, COVID-19 has spread…
Q: Human Immunodeficiency Virus (HIV) Principle: The assay starts with a sample applied to the sample…
A: Immunodiagnostic tests help in the diagnosis of disease with accuracy. This is because antibodies…
Q: During nucleic acid hybridization, the probe is labelled Question 1 options: for DNA stability to…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: ELISA is an example of a(n): A) enzyme assay. B) biological assay. C) binding assay. D)…
A: ELISA is used for detection of various targets. It is often used for diagnostic and research…
Q: Explain how COVID-19 can be detect using molecular based
A: Given: Need to find how the COVID-19- can be detected using molecular based technology. Coronavirus…
Q: Explain how viruses can be isolated using filtration State that viruses must be cultured in cells.…
A: Isolation of virus in the liquid medium It is easily done by centrifugation During this the viral…
Q: Briefly describe the principle of the MTT assay and how this assay helps you determine the LC50 of…
A: Testing cytotoxicity of a specific chemical or drug is a routine procedure in several biochemistry…
Q: Mass spectrometry is a powerful tool in proteomics. What are the four key features of a mass…
A: Mass spectrometry is a technique for determining the mass of individual biomolecules. Ionization,…
Q: Briefly describe how toe Bradford protein assay works
A: Introduction There are several methods to determine various biomolecules either quantitatively or…
Q: What data have been complied in the Human Protein Atlas? https://www.proteinatlas.org/about A.The…
A: The Human Protein Atlas is an extensive database used for wide distribution of proteins, which are…
Q: Match the types of data to the example.…
A: Precise measurements - I. Addition of 100 microlitres of standard to 3mL of Bradford reagent…
Q: pharmacogenomics a
A: introduction Pharmacogenomics is the investigation of what qualities mean for an individual's…
Q: Explain how antibodies can be used as tools for protein identification, purification, and…
A: Introduction: Immunological techniques begin with the production of antibodies against a specific…
Q: Describe the use of stem cells in medicine and cosmetology, analyze the advantages and disadvantages…
A: The use of stem cells to treat or prevent a disease or condition is known as stem-cell therapy.…
Q: Use at least 2 sources of information, which you can document using any traditional scheme of…
A: Hello. Since your question has multiple parts, we will solve first question for you. If you want…
Q: Questión 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes,…
A: Enzyme-linked Immunosorbent assay (ELISA) is a method of target antigen or antibody capture in…
Q: Please describe the CHIP assay. Why it is used? Please give examples Cells Lysis Cross-linking…
A: Chromatin immunoprecipitation (ChIP) assays This assay identifies the link between the genome and…
Q: Discuss briefy how a probe is used in molecular diagnostics.
A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…
Q: When would you use Edman degradation method and mass spectrometry in protein sequencing experiment
A: Edman degradation is a process of purifying protein.During the process of purification, protein gets…
Q: In size exclusion chromatography, which component will elute last in the set up? O Small proteins…
A: Size exclusion chromatography is a process of separation of molecules based on their size. The…
Q: Discuss the advantages of the genetic analysis of bacteria. Make alist of the types of allelic…
A: Bacteria are the single celled prokaryotic organisms that are found on most of the environmental…
Q: 9. Sequencing In order to ensure a reliable result, arrange the following procedure with A being the…
A: Mentioned steps of the procedure are performed to analyze the number of shoots and roots of onion.…
Q: Experiment: Bradford protein assay give the answers (4-5 lines) of review questions in the end.…
A: Dear students, We’ll answer the first question since the exact one wasn’t specified. Please submit…
Discuss the applications of molecular diagnostic tests
+++ pls make it organize and detail
Step by step
Solved in 3 steps
- When it comes to BC/DR preparation, why is testing so important? To what extent are there several sorts of examinations?Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.List 4 advantages of biological value of protein determination method.
- With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.What are the advantages of molecular diagnostics over conventional methods?Provide detailed explanation of the following super-resolution techniques: GSD, RESOLFT, SPDM, PAINT or DNA-PAINT.
- a. Briefly discuss (using three sentences) how the concepts and/or techniques in molecular biology are applied in COVID-19 pandemic, and: b. For each topic (Covid-19 pandemic), give any issues, benefits, or drawbacks of the application (still using three sentences).Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?Immunofluorescence and Fluorescent livecell imaging techniques can both be used to determine protein localisation.List the advantages and disadvantages of using immunofluorescence andfluorescent live cell imaging
- Compare the differences in the techniques used in whole genome shotgun sequencing and next generation sequencing. Use 5 to 10 intelligisentences only.Explain the significance of DNA isolation in cell and molecular biology experiments.Discuss the advantages of the genetic analysis of bacteria. Make alist of the types of allelic differences among bacteria that are suitablefor genetic analyses.