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Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk contrast.
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- Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?Explain in detail simple and differential staining and also improving and adjusting contrast in light microscopy.What is the advantage of phase-contrast microscopyover bright-field microscopy?
- What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy?How can bright field and dark field optical microscopy be used in characterizing materials? What kind of contrast is used in these techniques?
- In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.Compare and contrast how dark-field, phase-contrast, differential interference contrast, epifluorescence, and confocal microscopes work, and the kind of image provided by each. Give a specific use for each type.How does a phase-contrast microscope increase contrast?
