Simple staining is often necessary to improve contrast in which microscope? O fluorescence microscope O clectron microscope O darkfield microscope compound light microscope O phase-contrast microscope
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Compound light microscope and other light microscopes require staining of the specimen for identification of the specimen. These microscopes lack contrast and various parts of the microorganisms like flagella appear colourless in these microscope.
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- What is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-ProbeAs with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentationWhat part of the microscope adjusts the amount of light projected onto the slide? Group of answer choices A. rheostat B. on/off switch C. iris D. course adjustment
- A microscope that maintains focus when the objective magnification is increased is calleda. binocular.b. myopic.c. parfocal.d. refractive.e. resolute.WHAT ARE THE DIFFERENT TYPES OF MICROSCOPES AND THEIR USES? COMPOUND MICROSCOPEBRIGHT FIELD MICROSCOPEDARK FIELD MICROSCOPEPHASE CONTRAST MICROSCOPE DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPE (DIC)FLUORESCENCE MICROSCOPEELECTRON MICROSCOPESCANNING PROBE MICROSCOPEDIGITAL MICROSCOPEWhat are the corresponding ring colors of the objective lenses of the microscope? Scanner _________________________ Low Power Objective (LPO) _________________________ High Power Objective (HPO) _________________________ Oil immersion Objective (OIO) _________________________
- During endoscope staining method we use the boiling of water on a tripod technique. WHY?What is the function of the microscope diaphragm? source of light condenses light from the light source into a narrow beam regulates the amount of light passing through the specimen used to focus the specimen holds the objective lensesHow does the compound microscope achieve magnification? resolution? Cite your reference/s if possible.
- When trying to focus an object on the microscope, you always begin with the ________________ objective lens and use __________________ adjustment knob to bring the object in focus. Once object is focused, high power objective lens can be used to magnify the object, and only use the ___________________ adjustment knob to focus at this point. a scanning; coarse; fine b low power; coarse; fine c low power; fine; coarse d scanning, fine; coarseWhich of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the centerDefine the following terms:a.Simple stainingb.Complex mediumc.Defined (synthetic) mediumd.Parfocale.Negative stainingf. Resolving powerg.Magnifying power